Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11205-CD
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD43 protein
Cynomolgus Monkey CD43 (Asp20-Thr258) Accession # EHH60309.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp20
Predicted Molecular Mass
50 kDa
SDS-PAGE
106-118 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD-43 Fc Chimera (Catalog # 11205-CD) is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human Siglec-1 Protein (Catalog # 5197-SL) binds with an ED50 of 1.50-7.50 μg/mL.
When Recombinant Cynomolgus Monkey CD-43 Fc Chimera (Catalog # 11205-CD) is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human Siglec-1 Protein (Catalog # 5197-SL) binds with an ED50 of 1.50-7.50 μg/mL.
Scientific Data Images for Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF
Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein (Catalog # 11205-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 210-240 kDa, respectively.Formulation, Preparation and Storage
11205-CD
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD43
References
- Santamaría, M. et al. (1996) Cancer Res. 56:3526.
- Manjunath, N. et al. (1995) Nature 377:535.
- Brown, T.J. et al. (1996) J. Biol. Chem. 271:27686.
- Tuccillo, F.M. et al. (2014) BioMed Res International. https://doi.org/10.1155/2014/742831.
- Fuhlbrigge, R.C. et al. (2006) Blood 107:1421.
- Matsumoto, M. et al. (2005) J. Immuno. 175:8042.
- Yang, R.Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
- van den Berg, T.K. et al. (2001) J. Immuno. 166:3637.
- Moreno-Amaral, A.N. et al. (2012) J. Leukoc. Bio. 91:469.
- Sanchez-Mateos, P. et al. (1995) Blood, 86:2228.
- Hirano, K. et al. (2005) J. Biol. Chem. 280:39284.
- Rosenstein, Y. et al. (1991) Nature 354:233.
- Fiume, G. et al. (2013) PLoS ONE 8:e66087.
- Fiume, G. et al. (2012) Nucleic Acids Res. 40:3548.
- Andersson, C.C. et al. (2005) Biochem. J. 387:377.
- Santana, M.A. et al. (2000) J Biol. Chem. 275:31460.
Long Name
Cluster of Differentiation 43
Alternate Names
CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN
Gene Symbol
SPN
UniProt
Additional CD43 Products
Product Documents for Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF
For research use only
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