Recombinant Cynomolgus/Rhesus VSIG8 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11172-VS

R&D Systems, part of Bio-Techne
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11172-VS-050
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Key Product Details

Source

CHO

Accession #

XP_028702448.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived VSIG8 protein
Cynomolgus Monkey/Rhesus Macaque VSIG8
(Val24-Gly265)
Accession # XP_028702448.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val24

Predicted Molecular Mass

54 kDa

SDS-PAGE

56-63 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 0.500-10.0 μg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus VSIG8 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque VSIG8 Fc Chimera Protein Bioactivity.

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect 0.500-10.0 μg/mL.

Recombinant Cynomolgus Monkey/Rhesus Macaque VSIG8 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque VSIG8 Fc Chimera Protein (Catalog # 11172-VS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-63 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

11172-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Based on its similarity with human VSIG8, cynomolgus VSIG8 is predicted to consist of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, cynomolgus VSIG8 shares 99% and 100% aa identity with human and rhesus VSIG8, respectively.  VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

UniProt

XP_028702448.1

Additional VSIG8 Products

Product Documents for Recombinant Cynomolgus/Rhesus VSIG8 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus/Rhesus VSIG8 Fc Chimera Protein, CF

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