Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9864-SL

R&D Systems, part of Bio-Techne
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9864-SL-050
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Key Product Details

Source

HEK293

Accession #

EHH59463

Conjugate

Unconjugated

Applications

Bioactivity

View all Siglec-2/CD22 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey Siglec-2/CD22 protein
Asp20-Arg687, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

76 kDa

SDS-PAGE

100-113 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965.
The ED50 for this effect is 0.07-0.42 µg/mL.

Reviewed Applications

Read 3 reviews rated 4.7 using 9864-SL in the following applications:

Scientific Data Images for Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein, CF

Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein Bioactivity

Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein Bioactivity

Immobilized Recombinant Cynomolgus Monkey Siglec‑2/CD22 (Catalog # 9864-SL) supports the adhesion of human red blood cells. The ED50 for this effect is 0.07-0.42 μg/mL.
Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein SDS-PAGE

Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Siglec‑2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~110 kDa.

Formulation, Preparation and Storage

9864-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-2/CD22

Siglecs are sialic acid specific I‑type lectins that are characterized by an extracellular domain (ECD) with an N‑terminal Ig‑like V-type domain followed by varying numbers of Ig‑like C2-type domains (1, 2). Siglec-2, also known as B cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. In humans, two distinct Siglec-2 cDNAs that arise from differential RNA processing of the same gene have been isolated. The predominant Siglec-2 (Isoform CD22-beta) encodes an 847 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with four immunoreceptor tyrosine-based inhibition motifs (ITIMs) (3). The variant Siglec-2 (Isoform CD22-alpha) encodes a 647 aa polypeptide missing two Ig-like C2-type domains and has a truncated (23 aa) cytoplasmic tail (4). Within the ECD, cynomolgus Siglec-2 shares 85%, and 55% aa sequence identity with human and mouse Siglec-2, respectively. Siglec-2 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of Siglec-2 with trans ligands on opposing cells is found to be favored over the binding of ligands in cis (5). Consistent with a single ligand-binding region, the first two N-terminal Ig-like domains mediated CD22 adhesion with lymphocytes, neutrophils, monocytes, and erythrocytes (6). Besides its role as an adhesion molecule, Siglec-2 is a co-receptor that physically interacts with B cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation. It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling (7, 8).

References

  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  3. Wilson, G.L et al. (1991) J. Exp. Med. 173:137.
  4. Stamenkovic, I. and B. Seed (1990) Nature 345:74.
  5. Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.
  6. Engel, P. et al. (1995) J Exp Med. 181:1581
  7. Ravetch, J.V. and L.L. Lanier (2000) Science 290:84.
  8. Wienands, Y.J. et al. (1999) J. Biol. Chem. 274:18769.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Entrez Gene IDs

933 (Human); 12483 (Mouse); 308501 (Rat); 102130968 (Cynomolgus Monkey)

Gene Symbol

CD22

UniProt

EHH59463

Additional Siglec-2/CD22 Products

Product Documents for Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein, CF

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