Recombinant E. coli N-Acetyl-D-Glucosamine Kinase/NAGK, CF
R&D Systems, part of Bio-Techne | Catalog # 8020-GK
Key Product Details
Product Specifications
Source
E. coli-derived e. coli N-Acetyl-D-Glucosamine Kinase/NAGK protein
Met1-Asp303 with C-terminal 6-His tag
Met1-Asp303 with C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
34 kDa
SDS-PAGE
37-38 kDa, reducing conditions
Activity
Measured by its ability to phosphorylate N-acetyl-D-glucosamine.
The specific activity is >10,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >10,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
8020-GK
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol, Brij-35 and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: N-Acetyl-D-Glucosamine Kinase/NAGK
References
- Plumbridge, J. (2009). J. Bacteriol. 191:5641.
- Reith, J. et al (2011). J. Bacteriol. 193:5386.
- Uehara, T. and Park, J.T.(2011) J. Bacteriol. 186:7273.
- Wu, Z.L. (2011) PLoS One 6:e23172.
Long Name
N-Acetyl-D-Glucosamine Kinase
Alternate Names
GlcNAc Kinase, GNK, N-Acetylglucosamine Kinase
Gene Symbol
NAGK
UniProt
Additional N-Acetyl-D-Glucosamine Kinase/NAGK Products
Product Documents for Recombinant E. coli N-Acetyl-D-Glucosamine Kinase/NAGK, CF
Product Specific Notices for Recombinant E. coli N-Acetyl-D-Glucosamine Kinase/NAGK, CF
For research use only
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