Recombinant Human ACLP His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6425-AC

ACLP activates canonical WNT/Beta-catenin signaling through Frizzeld-8 and LRP-6
R&D Systems, part of Bio-Techne
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6425-AC-050
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Key Product Details

Source

HEK293

Accession #

Q8IUX7

Conjugate

Unconjugated

Applications

Bioactivity

View all ACLP Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ACLP protein
Gln26-Phe1158, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln26 inferred from enzymatic pyroglutamate treatment revealing Thr27.

Predicted Molecular Mass

130 kDa

SDS-PAGE

160-180 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LRP-6 Fc Chimera (Catalog # 1505-LR) is coated at 2 ug/mL, 100 uL/well, Recombinant ACLP binds with an ED50 of 1-8 μg/mL.

Scientific Data Images for Recombinant Human ACLP His-tag Protein, CF

Recombinant Human ACLP His-tag Protein Binding Activity

Recombinant Human ACLP His-tag Protein Binding Activity

When Recombinant Human LRP-6 Fc Chimera (Catalog # 1505-LR) is coated at 2 µg/mL, 100 µL/well, Recombinant Human ACLP (Catalog # 6425-AC) binds with an ED50 of 1-8 µg/mL.
Recombinant Human ACLP His-tag Protein SDS-PAGE

Recombinant Human ACLP His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ACLP His-tag (Catalog# 6425-AC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 160-180 kDa.

Formulation, Preparation and Storage

6425-AC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACLP

Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1) (1, 2), is a secreted ECM-associated protein expressed primarily by perivascular and vascular cells and is up-regulated in activated vascular cells following vascular injury (3). Pro-ACLP is composed of an N-terminal signal sequence that is cleaved off in the mature form, a Lys/Pro rich motif (aa 47-326), a Glu rich motif (aa 1079-1136), a collagen binding discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942) (4, 5). Mature human ACLP shares 84% aa sequence identity with mouse and rat ACLP. In the liver, ACLP is specifically expressed in hepatic stellate cells (HSCs). Although unrelated to WNT proteins, ACLP can specifically bind to Frizzled-8 and Lrp6 to form a ternary complex to activate the canonical WNT pathway and exacerbates nonalcoholic steatohepatitis (NASH) pathology, indicating that NASH can be treated by targeting ACLP (6). Diseases associated with ACLP-1 include Ehlers-Danlos syndrome classic-like 2 (EDSCLL2) (7) and gastroschisis. Over-expression of ACLP-1 is associated with glioblastoma (8).

References

  1. He, G.P. et al.(1995) Nature. 378: 92.
  2. Ro, H.S. et al. (2001) Gene. 280:123.
  3. Layne, M.D. et al. (2002) Cir. Res. 90:728.
  4. Tumelty, K.E. et al. (2014) J. Biol. Chem. 289:2526.
  5. Layne, M.D. et al. (1998) J. Biol. Chem. 273:15654.
  6. Teratani, T. et al. (2018) J Clin Invest. 128:1581.
  7. Blackburn, P.R. et al. (2018) Am J Hum Genet. 102:696.
  8. Ladha, J. et al. (2012) Mol Cancer Res. 10:1039.

Long Name

Aortic Carboxypeptidase-like Protein

Alternate Names

AEBP1

Entrez Gene IDs

165 (Human)

Gene Symbol

AEBP1

UniProt

Q8IUX7

Additional ACLP Products

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Product Specific Notices for Recombinant Human ACLP His-tag Protein, CF

For research use only

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