Recombinant Human Active MEK2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2855-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human MEK2 protein
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N terminal GST tag
SDS-PAGE
71 kDa
Activity
The specific activity of MEK2 was determined to be 190 nmol/min/mg in a coupled assay using an ERK2 substrate and a myelin basic protein (MBP) substrate.
Reviewed Applications
Read 1 review rated 4 using 2855-KS in the following applications:
Scientific Data Images for Recombinant Human Active MEK2 Protein, CF
Recombinant Human Active MEK2 Protein SDS-PAGE.
The approximate molecular weight is 71 kDa and the purity is > 80%.Formulation, Preparation and Storage
2855-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: MEK2
MEK2 is the member of MAPK kinase (MAPKK) family of signaling protein kinases. MEK2 is a dual-specificity kinase that activates the extracellular signal-regulated kinase (ERK) and mitogen-activated protein (MAP) kinase upon agonist binding to receptors. MEK2 plays a key role in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathways (1). Approximately 30% of all human cancers have a constitutively activated MAPK pathway, and constitutive activation of MEK2 results in cellular transformation. The ERK/MAP kinase cascade regulates cell growth and differentiation (2).
References
- Shuichan, X. et al. (1997) Mol. Endocrinol. 11:1618.
- Louis-François, B. et al. (2003) Mol. Cell. Biol. 23:4778.
Long Name
Mitogen-activated Protein Kinase Kinase 2
Alternate Names
MAP2K2, MKK2
Gene Symbol
MAP2K2
UniProt
Additional MEK2 Products
Product Documents for Recombinant Human Active MEK2 Protein, CF
Product Specific Notices for Recombinant Human Active MEK2 Protein, CF
For research use only
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