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Recombinant Human Active NEK2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3706-KS

R&D Systems, part of Bio-Techne
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3706-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human NEK2 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal GST tag

SDS-PAGE

76 kDa

Activity

The specific activity of NEK2 is typically 237-321 nmol/min/mg using a myelin basic protein (MBP) substrate.

Scientific Data Images for Recombinant Human Active NEK2 Protein, CF

Recombinant Human Active NEK2 Protein SDS-PAGE

Recombinant Human Active NEK2 Protein SDS-PAGE.

The approximate molecular weight is 76 kDa and the purity is > 90%.

Formulation, Preparation and Storage

3706-KS
Formulation Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: NEK2

NEK2 is closely related in its catalytic domain to the Serine/Threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2 (1). NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus (2).

References

  1. Schultz, S.J. et al. (1994) Cell Growth Differ. 5:625.
  2. Fry, A.M. et al. (1995) J. Biol. Chem. 270:12899.

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Entrez Gene IDs

4751 (Human); 18005 (Mouse); 114482 (Rat)

Gene Symbol

NEK2

Additional NEK2 Products

Product Documents for Recombinant Human Active NEK2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active NEK2 Protein, CF

For research use only

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