Skip to main content

Recombinant Human Active PDK-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 864-KS

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
864-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PDK-1 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal His tag

SDS-PAGE

67 kDa

Activity

The specific activity of PDK-1 was determined to be 102 nmol/min/mg using a synthetic peptide substrate.

Scientific Data Images for Recombinant Human Active PDK-1 Protein, CF

Recombinant Human Active PDK-1 Protein SDS-PAGE

Recombinant Human Active PDK-1 Protein SDS-PAGE

The approximate molecular weight is 67 kDa and the purity is > 95%.

Formulation, Preparation and Storage

864-KS
Formulation Supplied in 50 mM sodium phosphate (pH 7.0), 300 mM NaCl, 0.25 mM DTT, 150 mM imidazole, 0.1 mM PMSF, and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to one year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 (1). PDK‑1 then activates protein kinase B (PKB) that, in turn, inactivates glycogen synthase kinase-3 (GSK-3). The phosphorylation of other proteins by PKB and GSK-3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK‑1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4,5)P3 and/or PtdIns(3,4)P2. Human PDK‑1 is a 556 amino acid residue monomeric enzyme comprised of a catalytic domain that is most similar to the PKA, PKB, and PKC subfamily of protein kinases.

References

  1. Cohen, P. et al. (1997) FEBS Letter 410:3.
  2. Alessi, D.R. et al. (1997) Current Biol. 7:261.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Entrez Gene IDs

5170 (Human); 18607 (Mouse); 81745 (Rat)

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for Recombinant Human Active PDK-1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active PDK-1 Protein, CF

For research use only

Loading...
Loading...
Loading...