Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 637-AR

R&D Systems, part of Bio-Techne
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637-AR-100
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Key Product Details

Source

NS0

Accession #

Q53SV1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all Activin RIA/ALK-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Activin RIA/ALK-2 protein
Human Activin RIA
(Asp23-Val124 & Phe120-Val124)
Accession # Q53SV1		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23 & Phe120

Predicted Molecular Mass

39 kDa & 28 kDa (monomers)

SDS-PAGE

40-45 kDa & 34-38 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human BMP-6 (Catalog # 507-BP) is used at 0.25 μg/mL, the concentration of Recombinant Human Activin RIA/ALK-2 Fc Chimera that produces 50% of the optimal binding response is found to be approximately
1-5 μg/mL.

Formulation, Preparation and Storage

637-AR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIA/ALK-2

Activin RIA, also known as ALK-2, TSK-7L, SKR1, TSR-I, and ACTR-I, is a glycosylated 65 kDa type I receptor in the TGF-beta serine/threonine kinase receptor family (1-3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis
(1, 4-7). Mature human Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Ser-rich GS box and the kinase domain (8, 9). Within the ECD, human Activin RIA shares 99% and 94% aa sequence identity with mouse and rat Activin RIA, respectively. It shares 20%-26% aa sequence identity with other human type I receptors Activin RIB, BMPR-IA, BMPR-IB, and TGF-beta RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGM-B/DRAGON, a BMP coreceptor that also associates with other type I and type II receptors (10). Activin RIA can additionally phosphorylate the coreceptor Endoglin and is required for the inhibitory effect of Endoglin on prostate cancer cell motility (11).

References

  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. Chuva de Sousa Lopes, S.M. et al. (2004) Genes Dev. 18:1838.
  5. Zhang, D. et al. (2003) J. Bone Miner. Res. 18:1593.
  6. van Dinther, M. et al. (2010) J. Bone Miner. Res. 25:1208.
  7. Wang, J. et al. (2005) Dev. Biol. 286:299.
  8. Matsuzaki, K. et al. (1993) J. Biol. Chem. 268:12719.
  9. Attisano, L. et al. (1993) Cell 75:671.
  10. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  11. Romero, D. et al. (2010) Carcinogenesis 31:359.

Long Name

Activin Receptor IA

Alternate Names

ActivinRIA, ACVR1, ALK-2, ALK2

Entrez Gene IDs

90 (Human); 11477 (Mouse)

Gene Symbol

ACVR1

UniProt

Q53SV1

Additional Activin RIA/ALK-2 Products

Product Documents for Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

For research use only

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