Recombinant Human ADAM15 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9234-AD

R&D Systems, part of Bio-Techne
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9234-AD-050
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Key Product Details

Source

CHO

Accession #

Q13444

Conjugate

Unconjugated

Applications

Bioactivity

View all ADAM15 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAM15 protein
Asp207-Ser693 with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp207

Predicted Molecular Mass

53 kDa

SDS-PAGE

67-79 kDa, reducing conditions

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
The ED50 for this effect is 0.5-3 μg/mL

Scientific Data Images for Recombinant Human ADAM15 Protein, CF

Recombinant Human ADAM15 Protein Bioactivity

Recombinant Human ADAM15 Protein Bioactivity

Immobilized Recombinant Human ADAM15 (Catalog # 9234-AD) supports the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.5-3 μg/mL.

Formulation, Preparation and Storage

9234-AD
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM15

A disintegrin and metalloproteinase 15 (ADAM15), also known as MDC15 and Metargin, is an approximately 110 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM15 is synthesized with a 189 amino acid (aa) propeptide that contains a cysteine switch motif. The 75 kDa activated form of human ADAM15 (after removal of the propeptide) consists of a 490 aa extracellular domain (ECD) with a peptidase, disintegrin, cysteine-rich, and EGF-like domain followed by a 21 aa transmembrane segment and a 146 aa cytoplasmic domain (2). Within the ECD, human ADAM15 shares 85% aa sequence identity with mouse and rat ADAM15. Alternative splicing generates multiple additional isoforms with various deletions or substitutions in the cytoplasmic domain, deletion in the propeptide and peptidase domains, or truncation at the beginning of the EGF-like domain (3, 4). ADAM15 is widely expressed, including on colonic epithelial cells, smooth muscle cells, vascular endothelial cells, and it is upregulated during chronic inflammation and tumor progression (5-8). It is also expressed on spermatocytes where both propeptide and the protease domain are cleaved during spermatocyte maturation (9, 10). ADAM15 promotes endothelial permeability, T cell adhesion, MMP-9 production and activation, and tumor cell metastasis (6, 8, 11, 12). It binds to Integrin  alphaV  beta3 (13) and mediates the proteolytic shedding of cell surface
N-Cadherin, E-Cadherin, MICB, CD23/Fc epsilon RII, and FGF R2 (IIb) (6, 7, 14-16).

References

  1. Edwards, D.R. et al. (2008) Mol. Aspects Med. 29:258.
  2. Kratzschmar, J. et al. (1996) J. Biol. Chem. 271:4593.
  3. Kleino, I. et al. (2009) J. Cell. Biochem. 108:877.
  4. Kleino, I. et al. (2007) BMC Mol. Biol. 8:90.
  5. Mosnier, J.F. et al. (2006) Lab. Invest. 86:1064.
  6. Najy, A.J. et al. (2008) Cancer Res. 68:1092.
  7. Duan, X. et al. (2013) Mol. Med. Rep. 7:991.
  8. Dong, D.D. et al. (2015) Oncol. Rep. 34:2451.
  9. Pasten-Hidalgo, K. et al. (2008) Reproduction 136:41.
  10. Pasten, K. et al. (2014) Reproduction 148:623.
  11. Sun, C. et al. (2010) Cardiovasc. Res. 87:348.
  12. Charrier, L. et al. (2007) J. Biol. Chem. 282:16948.
  13. Zhang, X.P. et al. (1998) J. Biol. Chem. 273:7345.
  14. Najy, A.J. et al. (2008) J. Biol. Chem. 283:18393.
  15. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  16. Maretzky, T. et al. (2009) Biochem. J. 420:105.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Entrez Gene IDs

8751 (Human); 11490 (Mouse)

Gene Symbol

ADAM15

UniProt

Q13444

Additional ADAM15 Products

Product Documents for Recombinant Human ADAM15 Protein, CF

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Product Specific Notices for Recombinant Human ADAM15 Protein, CF

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