Recombinant Human ADAM22 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4908-AD

R&D Systems, part of Bio-Techne
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4908-AD-050
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Key Product Details

Source

CHO

Accession #

Q9P0K1

Structure / Form

Mixture of pro and mature forms

Conjugate

Unconjugated

Applications

Bioactivity

View all ADAM22 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAM22 protein
Gly26-His528 (Leu78Val), with a C-terminal 8-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26 & Asn233

Predicted Molecular Mass

58 kDa (pro) & 34 kDa (mature)

SDS-PAGE

40-45 kDa & 69-80 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 20-100 ng/mL.

Formulation, Preparation and Storage

4908-AD
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM22

A disintegrin and metalloproteinase 22 (ADAM22), also known as MDC2, is an 85-90 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM22 contains an N-terminal 197 amino acid (aa) cleavable proregion and a 514 aa extracellular domain (ECD). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, a cysteine‑rich region, and one EGF-like domain (2). The cytoplasmic portion contains two phosphorylation motifs which bind to 14-3-3 proteins to mediate cell adhesion (3, 4). Alternative splicing generates multiple isoforms with a variety of insertions, deletions, and substitutions in the cytoplasmic region (2, 5, 6). Within the peptidase-like and disintegrin domains, human ADAM22 shares 96% sequence identity with mouse and rat ADAM22. ADAM22 is expressed on neurons, astrocytes, and Schwann cells in the cerebral cortex, hippocampus, and cerebellum and also on some low-grade gliomas (5, 7-9). It can be up-regulated in endocrine therapy-resistant breast cancer, and it contributes to tumor cell migration (10). It mediates cell adhesion through binding to Integrins alpha6, alpha9, and beta3 (7). ADAM22 is localized to post-synaptic densities (11). It binds the secreted protein LGI1 which functions as a bridge to link ADAM22 with pre-synaptically expressed ADAM23 (9). The interaction of LGI1 with ADAM22 promotes AMPA receptor expression in hippocampal neurons and strengthens excitatory synapses (9). In axonal juxtaparanodes, ADAM22 is a component of the voltage-gated Kv1 potassium channel that responds to LGI1 (12). It also colocalizes with Kv1 channels in basket cell interneuron terminals of the cerebellum (12). ADAM22 additionally binds to Schwann cell derived LGI4 and is required for LGI4 to promote axon myelination (8). Mice lacking ADAM22 expression exhibit hypomyelination and profound ataxia (13).

References

  1. Edwards, D.R. et al. (2008) Mol. Aspects Med. 29:258.
  2. Sagane, K. et al. (1998) Biochem. J. 334:93.
  3. Godde, N.J. et al. (2006) J. Cell Sci. 119:3296.
  4. Zhu, P. et al. (2005) Biochem. Biophys. Res. Commun. 331:938.
  5. Harada, T. et al. (2000) Jpn. J. Cancer Res. 91:1001.
  6. Godde, N.J. et al. (2007) Gene 403:80.
  7. D’Abaco, G.M. et al. (2006) Neurosurgery 58:179.
  8. Ozkaynak, E. et al. (2010) J. Neurosci. 30:3857.
  9. Fukata, Y. et al. (2006) Science 313:1792.
  10. McCartan, D. et al. (2012) Cancer Res. 72:220.
  11. Fukata, Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:3799.
  12. Ogawa, Y. et al. (2010) J. Neurosci. 30:1038.
  13. Sagane, K. et al. (2005) BMC Neurosci. 6:33.

Long Name

A Disintegrin and Metalloproteinase Domain 22

Alternate Names

MDC2

Entrez Gene IDs

53616 (Human); 11496 (Mouse); 57033 (Rat)

Gene Symbol

ADAM22

UniProt

Q9P0K1

Additional ADAM22 Products

Product Documents for Recombinant Human ADAM22 Protein, CF

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Product Specific Notices for Recombinant Human ADAM22 Protein, CF

For research use only

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