Recombinant Human ADAMTS5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2198-AD
Key Product Details
Source
NS0
Accession #
Structure / Form
Recombinant Human ADAMTS5 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ADAMTS5 protein
Ser262-Pro622, with a C-terminal 10-His tag
Ser262-Pro622, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser262
Predicted Molecular Mass
41 kDa
SDS-PAGE
51 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Abz-TEGEARGSVI-Dap(Dnp)-KK-NH2.
The specific activity is >10 pmol/min/μg, as measured under the described conditions.
The specific activity is >10 pmol/min/μg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 4 using 2198-AD in the following applications:
Formulation, Preparation and Storage
2198-AD
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, CaCl2 and NaCl. |
| Reconstitution |
Reconstitute at 200 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ADAMTS5
ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha2-macroglobulin (3), aggrecan (4), and brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and rheumatoid arthritis. The purified rhADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and recombinant human TIMP-3 (Catalog # http://www.rndsystems.com/product_results.aspx?k=973-TM">973-TM).
References
- Porter, S. et al. (2005) Biochem. J. 386:15.
- Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
- Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554.
- Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259.
- Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239.
- Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
- Glasson, S.S. et al. (2005) Nature, 434: 644.
- Stanton, H. et al. (2005) Nature, 434:648.
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5
Alternate Names
ADAMTS11, ADMP-2, Aggrecanase 2
Gene Symbol
ADAMTS5
UniProt
Additional ADAMTS5 Products
Product Documents for Recombinant Human ADAMTS5 Protein, CF
Product Specific Notices for Recombinant Human ADAMTS5 Protein, CF
For research use only
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