Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9059-CL

R&D Systems, part of Bio-Techne
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9059-CL-050
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Key Product Details

Source

CHO

Accession #

Q92478

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all AICL/CLEC-2B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human AICL/CLEC-2B protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human AICL/CLEC-2B
(Lys26-His149) with a Met79Thr substitution
Accession # Q92478
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

41 kDa

SDS-PAGE

48-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human AICL/CLEC-2B Fc Chimera is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Galectin-1 (Catalog # 1152-GA) that produces 50% optimal binding response is 0.6-3 µg/mL.

Scientific Data Images for Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

Recombinant Human AICL/CLEC-2B Fc Chimera Protein Bioactivity

Recombinant Human AICL/CLEC-2B Fc Chimera Protein Bioactivity

When Recombinant Human AICL/CLEC-2B Fc Chimera (Catalog # 9059-CL) is coated at 2 µg/mL, Recombinant Human Galectin-1 (Catalog # 1152-GA) binds with a typical ED50 of 0.6-3 µg/mL.

Formulation, Preparation and Storage

9059-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AICL/CLEC-2B

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).

References

  1. Li, Y. et al. (2014) Front. Immunol. 5:123.
  2. Hamann, J. et al. (1997) Immunogenetics 45:295.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Klimosch, S.N. et al. (2013) Blood 122:2380.

Long Name

Activation-Induced C-Type Lectin

Alternate Names

CLEC-2B, CLEC2B, CLECSF2, IFNRG1

Entrez Gene IDs

9976 (Human)

Gene Symbol

CLEC2B

UniProt

Q92478

Additional AICL/CLEC-2B Products

Product Documents for Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

For research use only

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