Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11191

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q13740.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all ALCAM/CD166 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ALCAM/CD166 protein
Human ALCAM/CD166
(Trp28-Ala526)
Accession # Q13740.2		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp28

Predicted Molecular Mass

59 kDa

SDS-PAGE

71-90 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD6 Fc Chimera (Catalog # 627-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag (Catalog # AVI11191) binds with an ED50 of 20.0-200 ng/mL.

Scientific Data Images for Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein Binding Activity.

When Recombinant Human CD6 Fc Chimera (627-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein (Catalog # AVI11191) binds with an ED50 of 20.0-200 ng/mL.

Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein (Catalog # AVI11191) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-90 kDa.

Formulation, Preparation and Storage

AVI11191
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a surface glycoprotein belonging to the immunoglobulin superfamily (1, 2). ALCAM, along with MCAM and BCAM/Lu, form a small subgroup of adhesion molecules involved in tissue development and maintenance, neurogenesis, and regulation of immune responses (3). Mature human ALCAM consists of an extracellular domain (ECD) containing 2 IgV and 3 IgC domains, a transmembrane domain, and a short cytoplasmic domain (1, 2). Within the ECD, human ALCAM shares 93% amino acid sequence identity with both mouse and rat ALCAM. An isoform of ALCAM with a shorter stalk region in the ECD is known to exist and is associated with higher shedding tendency and decreased cell adhesion (2). Initially found expressed on activated leukocytes, ALCAM expression has been detected in a wide variety of tissues and cells (3, 4). In addition to cell adhesion, other ALCAM functions include leukocyte migration across the blood brain barrier, T-cell activation, and osteogenesis, (2, 5, 6). Overexpression of ALCAM is often associated with poor prognosis in various human tumors, such as bladder cancer, prostate cancer, melanoma, and liver cancer (2, 7). Our Avi-tag Biotinylated Human ALCAM/CD166 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Ferragut, F. et al. (2021) Cytokine Growth Factor Rev. 61:27.
  3. Swart, G.W. (2002) Eur. J. Cell Biol. 81:313.
  4. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  5. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  6. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  7. Darvishi, B. et al. (2020) Exp. & Mol. Path 115:104443.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Alternate Names

CD166

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Gene Symbol

ALCAM

UniProt

Q13740.2

Additional ALCAM/CD166 Products

Product Documents for Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein, CF

For research use only

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