Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF

R&D Systems, part of Bio-Techne | Catalog # 7529-DH

R&D Systems, part of Bio-Techne
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7529-DH-020
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

O60218

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Aldo-keto Reductase 1B10/AKR1B10 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Aldo-keto Reductase 1B10/AKR1B10 protein
Ala2-Tyr316, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments

Predicted Molecular Mass

37 kDa

SDS-PAGE

37-38 kDa, reducing conditions

Activity

Measured by the disappearance of NADPH during the reducation of 4-nitrobenzaldehyde.
The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 2 reviews rated 5 using 7529-DH in the following applications:

Formulation, Preparation and Storage

7529-DH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aldo-keto Reductase 1B10/AKR1B10

Aldo-keto reductase family 1 member B10 (AKR1B10), also known as ARL-1 or aldose reductase related protein, is a cytosolic, NADPH-dependent oxidoreductase that reduces variety of aromatic and aliphatic aldehydes, dicarbonyl compounds, and some drug ketones (1). It is related to AKR1B1 in amino acid sequence and tertiary structure. However, its substrate specificity differs from AKR1B1 in its inability to reduce sugars and prostaglandin H2, and in its high catalytic efficiency for retinals, isoprenyl aldehydes, and cytotoxic aldehydes (2). It is primarily observed in the human colon, small intestine, and adrenal gland, with a low level in liver. Its up‑regulated expression is detected in hepatocellular carcinoma, cervical cancer, lung squamous cell carcinoma, and lung adenocarcinoma in smokers (3). Thus, it is considered as a potential diagnostic and/or prognostic marker in carcinomas and serum. The functional studies of this enzyme suggest that the up‑regulation of AKR1B10 is related to cell survival by reducing chemo-agents or metabolites in cells to less toxic reduced forms (4). Its inhibition may be a potential target for reducing the progression of cancers (5).

References

  1. Endo, S. et al. (2009) Arch. Biochem. Biophys. 487:1.
  2. Gallego, O. et al. (2007) Proc. Natl. Acad. Sci. USA 104:20764.
  3. Matsunaga, T. et al. (2012) Front. Pharmacol. 3:1.
  4. Wang, C. et al. (2009) J. Biol. Chem. 284:26742.
  5. Matsunaga, T. et al. (2011) Anticancer Drugs 22:402.

Long Name

Aldo-keto Reductase Family 1, Member B10 [Aldose Reductase]

Alternate Names

AKR1B10, AKR1B11, AKR1B12, AldoketoReductase 1B10, ARL1, HIS, HSI

Entrez Gene IDs

57016 (Human); 67861 (Mouse); 296972 (Rat)

Gene Symbol

AKR1B10

UniProt

O60218

Additional Aldo-keto Reductase 1B10/AKR1B10 Products

Product Documents for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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