Recombinant Human Aldo-keto Reductase 1C4/AKR1C4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6957-DH
Key Product Details
Product Specifications
Source
E. coli-derived human Aldo-keto Reductase 1C4/AKR1C4 protein
Met1-Tyr323
Met1-Tyr323
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
37 kDa
SDS-PAGE
36-38 kDa, reducing conditions
Activity
Measured by its NADP+-dependent oxidation of cholic acid.
The specific activity is >300 pmol/min/μg, as measured under the described conditions.
The specific activity is >300 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6957-DH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aldo-keto Reductase 1C4/AKR1C4
Aldo-keto Reductase 1C4 (AKR1C4), also known as dihydrodiol dehydrogenase 4, is a member of the aldo-keto reductase (AKR) superfamily. It catalyzes the reversible oxidoreduction of various 3 alpha‑hydroxysteroids and prostaglandins as well as the oxidation of trans‑dihydrodiols of aromatic hydrocarbons and alicyclic alcohols (1‑4). AKR1C4 is important for steroid hormone metabolism primarily through synthesis and clearance of testosterone. Its high expression in liver and the ability to reduce xenobiotic carbonyl compounds suggests an important role in xenobiotic metabolism, as is manifested by the detoxification of the pesticide chlordecone in humans (5). AKR1C4 also plays a role in the pharmacokinetics of various drugs and prodrug activation (6, 7).
References
- Penning, P. M. et al. (1990) Steroids 47:221.
- Takikawa, H. et al. (1992) Hepatology 16:365.
- Binstock, J. M. et al. (1992) Biochem. Biophys. Res. Commun. 187:760.
- Ohara, H. et al. (1994) Biochim. Biophys. Acta. 1215:59.
- Winters, C. J. et al. (1990) Biochemistry 29:1080.
- Jin, Y. et al. (2009) J. Biol. Chem. 284:10013.
- Breyer-Pfaff, U. et al. (2004) J. Pharm. Pharmacol. 56:1601.
Long Name
Aldo-keto Reductase Family 1, Member C4
Alternate Names
3-alpha-HSD, AKR1C4, AldoketoReductase 1C4, CDR, CHDR, DD4, HAKRA
Entrez Gene IDs
1109 (Human)
Gene Symbol
AKR1C4
UniProt
Additional Aldo-keto Reductase 1C4/AKR1C4 Products
Product Documents for Recombinant Human Aldo-keto Reductase 1C4/AKR1C4 Protein, CF
Product Specific Notices for Recombinant Human Aldo-keto Reductase 1C4/AKR1C4 Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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