Recombinant Human AMIGO Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2565-AM
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human AMIGO protein
Gly28-His367, with a C-terminal 6-His tag
Gly28-His367, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Starts at Gly28
Predicted Molecular Mass
39.5 kDa
SDS-PAGE
50-65 kDa, reducing conditions
Activity
Measured by its ability to enhance survival of E16-E18 rat embryonic cortical neurons.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Optimal neurite outgrowth is observed between 1.25-10 μg/mL.
The ED50 for this effect is 0.6-2.4 μg/mL.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Optimal neurite outgrowth is observed between 1.25-10 μg/mL.
Formulation, Preparation and Storage
2565-AM
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: AMIGO
References
- de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
- Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
- Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
- Chen, Y. et al. (2012) J. Cell. Physiol. 227:2217.
- Peltola, M.A. et al. (2011) EMBO Rep. 12:1293.
- Park, K.-S. et al. (2006) Science 313:976.
Long Name
Amphoterin-induced Gene and ORF
Alternate Names
ali2, Alivin-2, AMIGO1
Gene Symbol
AMIGO1
UniProt
Additional AMIGO Products
Product Documents for Recombinant Human AMIGO Protein, CF
Product Specific Notices for Recombinant Human AMIGO Protein, CF
For research use only
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