Recombinant Human AMIGO2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2080-AM

R&D Systems, part of Bio-Techne
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2080-AM-050
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Key Product Details

Source

CHO

Accession #

Q86SJ2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all AMIGO2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human AMIGO2 protein
Human AMIGO2
(Gly39-His393)
Accession # Q86SJ2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly39 & Val40

Predicted Molecular Mass

66.9 kDa (monomer)

SDS-PAGE

90-110 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 0.5-2.0 μg/mL.

Formulation, Preparation and Storage

2080-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO2

AMIGO2 (amphoterin‑induced gene and ORF 2), also known as DEGA and Alivin‑1, is an approximately 65 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Mature human AMIGO2 consists of a 359 amino acid (aa) extracellular domain (ECD) with six LRRs flanked by single LRRNT and LRRCT domains, followed by one immunoglobulin-like domain, a 21 aa transmembrane segment, and a 103 aa cytoplasmic domain (3‑5). Within the ECD, human AMIGO2 shares 89% and 87% aa sequence identity with mouse and rat AMIGO2, respectively. AMIGO2 forms homodimers as well as heterodimers with the related AMIGO and AMIGO3 molecules (3, 6). Within the nervous system, AMIGO2 is transcribed in the cerebrum, hypothalamus, olfactory bulb, retina, hippocampus (pyramidal cells), and cerebellum (granule neurons and Purkinje cells) (3, 4, 7). It is also expressed in the liver, lung, testis, spleen, and small intestine (3, 4). AMIGO2 supports neuron survival, and is down‑regulated following pro‑apoptotic stimulation (4). Its expression can be up‑regulated or down‑regulated in a variety of cancers, and anti‑sense knockdown of AMIGO2 interferes with tumor cell adhesion to collagen as well as in vivo tumorigenicity (5).

References

  1. de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
  2. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
  3. Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
  4. Ono, T. et al. (2003) J. Neurosci. 23:5887.
  5. Rabenau, K.E. et al. (2004) Oncogene 23:5056.
  6. Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
  7. Laeremans, A. et al. (2013) Brain Struct. Funct. 218:123.

Long Name

Amphoterin-induced Gene and ORF 2

Alternate Names

Alivin-1

Entrez Gene IDs

347902 (Human); 105827 (Mouse); 102124161 (Cynomolgus Monkey)

Gene Symbol

AMIGO2

UniProt

Q86SJ2

Additional AMIGO2 Products

Product Documents for Recombinant Human AMIGO2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human AMIGO2 Fc Chimera Protein, CF

For research use only

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