Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2970-ZN
Key Product Details
Product Specifications
Source
E. coli-derived human Aminopeptidase P1/XPNPEP1 protein
Met1-His623 & Pro2-His623, both with a C-terminal 6-His tag
Met1-His623 & Pro2-His623, both with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1 & Pro2
Predicted Molecular Mass
71 kDa
SDS-PAGE
71 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)-Pro-Pro-p-Nitroanilide (K(Abz)PP-pNA).
The specific activity is >1,300 pmol/min/µg, as measured under the described conditions.
The specific activity is >1,300 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2970-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminopeptidase P1/XPNPEP1
The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1‑3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). The purified rhAPP1 is an active aminopeptidase, removing a N‑terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74% and 73% identical to that of canine, bovine, mouse/rat, Xenopus and zebrafish, respectively.
References
- Cottrell, G.S. et al. (2000) Biochemistry 39:15121.
- Sprinkle, T.J. et al. (2000) Arch. Biochem. Biophys. 378:51.
- Vanhoof, G. et al. (1997) Cytogenet. Cell Genet. 78:275.
- Turner, A.J. and G.S. Cottrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 931 - 933, Elsevier Academic Press, San Diego.
- Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934 - 937, Elsevier Academic Press, San Diego.
Alternate Names
APP1, SAMP, XPNPEP1
Entrez Gene IDs
7511 (Human)
Gene Symbol
XPNPEP1
UniProt
Additional Aminopeptidase P1/XPNPEP1 Products
Product Documents for Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
Product Specific Notices for Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
For research use only
Loading...
Loading...
Loading...