Recombinant Human Angiopoietin-like 3 (aa 17-220), CF

R&D Systems, part of Bio-Techne | Catalog # 4954-AN

R&D Systems, part of Bio-Techne
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4954-AN-050
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Key Product Details

Source

CHO

Accession #

Q9Y5C1

Structure / Form

Oligomer

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Angiopoietin-like Protein 3/ANGPTL3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 3/ANGPTL3 protein
Ser17-Pro220, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser17

Predicted Molecular Mass

24.6 kDa

SDS-PAGE

35-43 kDa, reducing conditions

Activity

Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase/LPL (Catalog # 9888-LL)  and p-nitrophenyl butyrate are present in 0.1 M Sodium Phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is approximately <15 µg/mL.

Formulation, Preparation and Storage

4954-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1-3). Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (4). Within the N-terminal region, human ANGPTL3 shares 15%-21% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 5, 6, and 7, and 82% with mouse and rat ANGPTL3. ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C-terminal segments containing the coiled coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly-associated, noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (7, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with integrin alphaV beta3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (16).

References

  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Trans. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
  10. Koster, A. et al. (2005) Endocrinology 146:4943.
  11. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  12. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  13. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  14. Matsusue, K. et al. (2006) Mol. Cell. Endocrinol. 256:23.
  15. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  16. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9Y5C1 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Recombinant Human Angiopoietin-like 3 (aa 17-220), CF

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