Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9606-AP

R&D Systems, part of Bio-Techne
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9606-AP-050
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Key Product Details

Source

E. coli

Accession #

P06727

Conjugate

Unconjugated

View all Apolipoprotein A-IV/ApoA4 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Apolipoprotein A-IV/ApoA4 protein
Glu21-Ser396, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

44 kDa

SDS-PAGE

43 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9606-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A-IV (ApoA4) is a 45 kDa glycoprotein of the lipid transport system. Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption. Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis (1). ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well (2). ApoA4 shares several structural characteristics with ApoA1 and other exchangeable apolipoproteins. The core domain of human ApoA4 contains thirteen 22-amino acid tandem repeats, and nine of which are predicted to be amphipathic alpha-helical repeats that are critical for lipid binding and self-association (3). The overall structure of a long rod‑like dimer consisting of two 4-helix bundles stacked end-to-end in opposing orientations (4). Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

References

  1. Duverger N. et al. (1991) Eur. J. Biochem. 201:373.
  2. Maeda, N. et al. (1994) J. Biol. Chem. 269:23610.
  3. Segrest, J. P. et al. (1994). Adv. Protein. Chem. 45:303.
  4. Deng, X. et al. (2012) Structure 20:767.

Alternate Names

Apolipoprotein AIV

Entrez Gene IDs

337 (Human); 11808 (Mouse); 25080 (Rat)

Gene Symbol

APOA4

UniProt

P06727

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

For research use only

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