Recombinant Human Arylsulfatase A/ARSA Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2485-SU
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Arylsulfatase A/ARSA protein
Arg19-Ala507, with a C-terminal 10-His tag
Arg19-Ala507, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg19
Predicted Molecular Mass
53 kDa
SDS-PAGE
63 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >25 pmol/min/µg, as measured under the described conditions.
The specific activity is >25 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2485-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Arylsulfatase A/ARSA
As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Recombinant human ARSA corresponds to the mature chain and has sulfatase activity described above.
References
- Lukatela, G. et al. (1998) Biochemistry 37:3654.
- Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
- Stein, C. et al. (1989) J. Biol. Chem. 264:1252.
Alternate Names
ARSA, Cerebroside 3-sulfatase
Gene Symbol
ARSA
UniProt
Additional Arylsulfatase A/ARSA Products
Product Documents for Recombinant Human Arylsulfatase A/ARSA Protein, CF
Product Specific Notices for Recombinant Human Arylsulfatase A/ARSA Protein, CF
For research use only
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