Recombinant Human AS3MT Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9827-MT

R&D Systems, part of Bio-Techne
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9827-MT-050
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Key Product Details

Source

E. coli

Accession #

Q9HBK9-1

Conjugate

Unconjugated

Applications

Bioactivity

View all AS3MT Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human AS3MT protein
Ala2-Cys375, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2 & Ala3

Predicted Molecular Mass

42 kDa

SDS-PAGE

41 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9827-MT
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AS3MT

Arsenite methyltransferase (AS3MT) is a cytosolic, 42 kDa enzyme that belongs to the methyltransferase superfamily and catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arsenite. Arsenic exposure in humans results in a broad range of acute and chronic life-long health risks including cancer, diabetes, cardiovascular and neurological diseases (1-4). Specifically, in all regions of the brain, arsenic exposure can cause a dose-dependent accumulation of arsenic compounds that interfere with glucose transporter function and cause a neurotoxic effect (5). In contrast, some arsenic forms and metabolites of arsenic are effective as therapeutic treatments for cancer (4, 6-8). In humans, AS3MT biomethylation paradoxically detoxifies arsenic and produces carcinogenic metabolites (4, 9-10). Several polymorphisms in the AS3MT gene have been shown to affect the protein's catalytic activity and result in potentially increased risk of arsenic-related disease (11). AS3MT is expressed primarily in liver although it is present in lower abundance in other tissues (12). Expression is also observed in plaque-resident cells where it contributes to atherosclerosis (13) and a truncated isoform of AS3MT is found to have increased expression in the brain where it is associated with schizophrenia (14, 15).

References

  1. Abernathy, C.O. et al. (2003) J. Nutr. 133:1536S.
  2. Tchounwou, P.B. et al. (2003) Toxicol. Pathol. 31:575.
  3. States, J.C. et al. (2009) Toxicol. Sci. 107:312.
  4. Khairul, I. et al. (2017) Oncotarget 8:23905.
  5. Rodriguez, V.M. et al. (2005) Toxicol. Sci. 84:157.
  6. Dilda, P.J. and P.J Hogg (2007) Cancer Treat. Rev. 33:542.
  7. Lalleman-Breitenback, V. et al. (2012) Trends. Mol. Med. 18:36.
  8. Lengfedler, E. et al. (2012) Leukemia. 26:433.
  9. Ajees, A.A. et al. (2012) Biochemistry. 51:5476.
  10. Dheeman, D.S. et al. (2014) Chem. Res. Toxicol. 27:1979.
  11. Li, J. et al. (2017) Chem. Res. Toxicol. 30:1481.
  12. Kobayashi, Y. et al. (2007) Environ. Toxicol. Pharmacol. 23:115.
  13. Negro Silva, L.F. et al. (2017) Environ Health Perspect. 125:077001.
  14. Li, M. et al. (2016) Nat. Med. 22:649.
  15. Li, L. et al. (2016) Mol. Neuropsychiatry 2:213.

Long Name

Arsenite Methyltransferase

Alternate Names

Arsenic (+3 Oxidation State) Methyltransferase, Arsenic Methyltransferase, CYT19, Methylarsonite Methyltransferase, Methyltransferase Cyt19, S-Adenosyl-L-Methionine:Arsenic(III) Methyltransfe, S-Adenosylmethionine:Arsenic (III) Methyltransfera

Entrez Gene IDs

57412 (Human); 57344 (Mouse); 140925 (Rat)

Gene Symbol

AS3MT

UniProt

Q9HBK9-1

Additional AS3MT Products

Product Documents for Recombinant Human AS3MT Protein, CF

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Product Specific Notices for Recombinant Human AS3MT Protein, CF

For research use only

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