Recombinant Human ASAM Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5019-AM

R&D Systems, part of Bio-Techne
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5019-AM-050
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Key Product Details

Source

CHO

Accession #

Q9H6B4

Conjugate

Unconjugated

Applications

Bioactivity

View all ASAM Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ASAM protein
Gly18-Met233, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly18

Predicted Molecular Mass

25.2 kDa

SDS-PAGE

33-37 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
When 4 x 104 cells/well are added to Recombinant Human ASAM coated plates (30 µg/mL, 100 µL/well), approximately 25%‑50% will adhere after one hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5019-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAM

Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and coxsackie- and adenovirus receptor-like membrane protein (CLMP), is a type I transmembrane protein and member of the CTX family within the immunoglobulin superfamily. Mature human ASAM consists of a 217 amino acid (aa) extracellular region, a 21 aa transmembrane region, and a 117 aa cytoplasmic tail. The extracellular region contains one V-type and one C-type immunoglobulin domain connected by a J segment (1, 2). Within the ECD, human ASAM shares 97% aa sequence identity with mouse and rat ASAM. ASAM is widely expressed as 44 kDa and 48 kDa species in epithelial tissues where it localizes to tight junctions (1, 3). It mediates cell‑cell adhesion and contributes to trans-epithelial resistance (1). It is down‑regulated in Sertoli cells by TNF-alpha which also inhibits the expression of several other tight junction proteins in seminiferous tubules (4, 5). ASAM is additionally expressed by mature adipocytes and is up‑regulated in white adipose tissue under conditions of obesity (2).

References

  1. Raschperger et al. (2004) J. Biol. Chem. 279:796.
  2. Eguchi, J. et al. (2005) Biochem. J. 387:343.
  3. Sze, K.-L. et al. (2008) J. Cell Physiol 214:334.
  4. Sze, K.-L. et al. (2008) Biochem. J. 410:575.
  5. Li, M.W. et al. (2006) J. Endocrinol. 190:313.

    Long Name

    Adipocyte-specific Adhesion Molecule

    Alternate Names

    ACAM, Asp5, CLMP

    Entrez Gene IDs

    79827 (Human); 71566 (Mouse); 286939 (Rat)

    Gene Symbol

    CLMP

    UniProt

    Q9H6B4

    Additional ASAM Products

    Product Documents for Recombinant Human ASAM Protein, CF

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    Product Specific Notices for Recombinant Human ASAM Protein, CF

    For research use only

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