Recombinant Human B3GAT1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8560-GT

R&D Systems, part of Bio-Techne
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8560-GT-050
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Key Product Details

Source

CHO

Accession #

Q9P2W7

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta-1,3-Glucuronyltransferase 1/B3GAT1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human beta-1,3-Glucuronyltransferase 1/B3GAT1 protein
His25-Ile334, with N-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

36 kDa

SDS-PAGE

50-59 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcA from UDP-GlcA to lactose.
The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8560-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta1-3Gal beta1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References

  1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
  2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
  3. Kakuda, S. et al. (2005) Glycobiology 2:203.
  4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
  5. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

Alternate Names

CD57, GLCATP, GLCUATP, HNK1, LEU7

Entrez Gene IDs

27087 (Human); 76898 (Mouse); 117108 (Rat)

Gene Symbol

B3GAT1

UniProt

Q9P2W7

Additional beta-1,3-Glucuronyltransferase 1/B3GAT1 Products

Product Documents for Recombinant Human B3GAT1 Protein, CF

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Product Specific Notices for Recombinant Human B3GAT1 Protein, CF

For research use only

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