Recombinant Human B3GNT6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6505-GT

R&D Systems, part of Bio-Techne
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6505-GT-020
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Key Product Details

Source

CHO

Accession #

Q6ZMB0

Conjugate

Unconjugated

Applications

Bioactivity

View all Beta-1,3-N-Acetylglucosaminyltransferase 6/B3GNT6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Beta-1,3-N-Acetylglucosaminyltransferase 6/B3GNT6 protein
Gln32-Ser384, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

40 kDa

SDS-PAGE

43-55 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcNAc from UDP-GlcNAc to 4-nitrophenyl-alpha -D-galactosaminide.
The specific activity is >35 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6505-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 6/B3GNT6

beta-1,3-N-acetylglucosaminyltransferase 6 (B3GNT6) is also known as core 3 synthase due to its role in synthesis of the core 3 structure (GlcNAc beta1‑3Gal‑NAc alpha1‑serine/threonine), an important precursor in the biosynthesis of mucin-type glycoproteins in digestive organs (1). Its expression is restricted to the stomach, colon and small intestine, where the core 3 structure is present. Down-regulation of the enzyme was found in gastric and colorectal carcinomas and it was suggested that it may be useful as a marker to distinguish between benign adenomas and premalignant lesions (2, 3). Prostate cancer cells transfected with core 3 synthase exhibited reduced migration and invasion compared with mock-transfected cells (4). When inoculated into nude mice, the transfected cells produced smaller tumors without metastasis in contrast to the robust tumor formation and metastasis observed in mock-transfected cells (4). Like other members of the beta‑1,3‑N‑acetylglucosaminyltransferase family, B3GNT6 is a Golgi-resident single-pass type II membrane protein.The activity of this enzyme has been measured with a phosphatase-coupled method (5).

References

  1. Iwai, T. et al. (2002) J. Biol. Chem. 277:12802.
  2. Iwai, T. et al. (2005) Proc. Natl. Acad. Sci. USA 102:4572.
  3. Vavasseur, F. et al. (1995) Glycobiology 5:351.
  4. Lee, S.H. et al. (2009) J. Biol. Chem. 284:17157.
  5. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

Alternate Names

Beta3Gn-T6, BGnT-6, Core 3 synthase

Entrez Gene IDs

192134 (Human)

Gene Symbol

B3GNT6

UniProt

Q6ZMB0

Additional Beta-1,3-N-Acetylglucosaminyltransferase 6/B3GNT6 Products

Product Documents for Recombinant Human B3GNT6 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human B3GNT6 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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