Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10381

R&D Systems, part of Bio-Techne
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AVI10381-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q5ZPR3.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Bioactivity

View all B7-H3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human B7-H3 protein
Human B7-H3 (4Ig)/B7-H3b
(Leu29-Thr461)
Accession # Q5ZPR3.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

75 kDa

SDS-PAGE

104-120 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 0.1-1 μg/mL.

Measured by its binding ability in a functional ELISA.
When Human B7-H3 Antibody (Catalog # AF1027) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant B7-H3 (4Ig)/B7-H3b Fc Chimera Avi-tag (Catalog # AVI10381)that produces 50% of the optimal binding response is approximately 10-60 ng/mL.

Scientific Data Images for Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF

Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein Binding Activity

Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein Binding Activity

When Human B7-H3 Antibody (Catalog # AF1027) is immobilized at 1.0 µg/mL, 100 µg/mL, the concentration of Biotinylated Recombinant B7-H3 (4Ig)/B7-H3b Fc Chimera Avi-tag (Catalog # AVI10381) that produces 50% the optimal binding response is approximately 10-60 ng/mL.
Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein SDS-PAGE

Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Avi-tag (Catalog # AVI10381) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 104-120 kDa and 208-240 kDa, respectively.

Formulation, Preparation and Storage

AVI10381
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H3

Human B7 homolog 3 (B7-H3), also known as CD276, is a member of the B7 family of immune checkpoint molecules, responsible for regulating immune responses (1‑3). There are at least seven structurally related B7 family members, all sharing about 20-40% amino acid (aa) sequence identity (4). The B7 protein family are immunoglobulin (Ig) superfamily members with varying numbers of Ig-V-like and Ig-C-like regions in the extracellular domain (ECD) and they can either be glycosylphosphatidylinositol (GPI)-linked or transmembrane (4). The mature ECD of B7-H3 contains two V-like and two C-like Ig domains, a transmembrane region, and a short cytoplasmic domain. An isoform of human B7-H3 containing only a single set of V-like and C-like Ig domains in the ECD has also been identified (1, 5). The ECD of both mouse and rat B7-H3 only contain a single set of V-like and C-like Ig domains. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines (1, 6). B7-H3 is also overexpressed in numerous cancers including bladder, breast and melanoma (7). Unlike other B7 family members, human B7-H3 does not bind any known members of the CD28 family of immunoreceptors and its receptor has yet to be identified. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to co-stimulate the proliferation of CD4+ or CD8+ T cells (8). B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1-3, 8).

References

  1. Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Collins M. et al. (2005) Genome Biol. 6:223.
  5. Ling, V. et al. (2003) Genomics. 82:365.
  6. Prasad, DV et al. (2004) J Immunol. 173:2500.
  7. Dong P. et al. (2018) Front Oncol. 8:264.
  8. Suh WK, et al. (2003) Nat Immunol. 4:899.

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Entrez Gene IDs

80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)

Gene Symbol

CD276

UniProt

Q5ZPR3.1

Additional B7-H3 Products

Product Documents for Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF

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