Recombinant Human BCAM His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11173-BC

R&D Systems, part of Bio-Techne
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11173-BC-050
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Key Product Details

Source

HEK293

Accession #

CAA58449.1

Conjugate

Unconjugated

Applications

Bioactivity

View all BCAM Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BCAM protein
Glu32-Ala547, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu32

Predicted Molecular Mass

57 kDa

SDS-PAGE

67-82 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of TE-85 human osteogenic sarcoma cells.
The ED50 for this effect is 0.250-3.00 μg/mL.

Scientific Data Images for Recombinant Human BCAM His-tag Protein, CF

Recombinant Human BCAM His-tag Protein Bioactivity.

Recombinant Human BCAM His-tag Protein (Catalog # 11173-BC) supports the adhesion TE‑85 human osteogenic sarcoma cells. The ED50 for this effect is 0.250-3.00 µg/mL.

Recombinant Human BCAM His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human BCAM His-tag Protein (Catalog # 11173-BC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-82 kDa.

Formulation, Preparation and Storage

11173-BC
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BCAM

Human Basal Cell Adhesion Molecule (BCAM), also known as CD239, is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu). Lu and BCAM differ by a 40 amino acid (aa) SH3-containing segment that is present in the cytoplasmic domain of Lutheran (1). Mature human BCAM consists of an extracellular domain (ECD) with two Ig-like V-type domains and three Ig-like C2-type domains, a transmembrane domain, and a short cytoplasmic domain (2,3). Within the ECD, human BCAM shares 73% amino acid (aa) identity with mouse and rat BCAM. A polymorphism at position 77 within the ECD is the basis for the difference between the Lua and Lub Lutheran blood groups (4). BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation (5-7). BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle (6,8,9). Lu/BCAM binds to laminin, specifically isoforms containing the alpha5 chain, which are found in basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation (10). Overexpression of both BCAM and Lu on sickle red blood cells (SS RBC) has been found to play a role in vaso-occlusive crisis in sickle cell patients by contributing to the adhesion of erythrocytes to the vascular wall (11,12). The adhesive role of Lu/BCAM has been studied in the context of many diseases, including sickle cell disease, hereditary spherocytosis, myeloproliferative neoplasms and Gaucher disease (13). BCAM is upregulated on carcinomas, sarcomas, astrocytomas, and melanomas (14). Additionally, Lu/BCAM has been found to assist tumor cell migration via regulation of integrin-mediated cell attachment to laminin-511 (15).

References

  1. Rahuel, C. et al. (1996) Blood 88:1865.
  2. Vainionpaa, N. et al. (2006) Am. J. Physiol. Cell Physiol. 290:C764.
  3. El Nemer, W. et al. (1997) Blood 89:4608.
  4. El Nemer, W. et al. (1999) J. Biol. Chem. 274:31903.
  5. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
  6. Rahuel, C. et al. (2008) Am. J. Physiol. Renal Physiol. 294:F393.
  7. Rettig, W.J. et al. (1986) Cancer Res. 46:6406.
  8. Nishimune, H. et al. (2008) J. Cell Biol. 182:1201.
  9. Kikkawa, Y. et al. (2007) J. Biol. Chem. 282:14853.
  10. El Nemer, W. et al. (2001) J Biol Chem 276:23757.
  11. Eyler, C.E. and Telen, M.J. (2006) Transfusion. 46(4).
  12. Klei, TRL. et al. (2018) Blood Adv. 2:14.
  13. Guadall, A. et al. (2019). J. Biol. Chem. 294:14911.
  14. Chang, H.Y. et al. (2017) J. Biomed Sci 24:61.
  15. Kikkawa, Y. et al. (2013) J. Biol. Chem. 288:30990.

Long Name

Basal Cell Adhesion Molecule

Alternate Names

CD239

Entrez Gene IDs

4059 (Human); 57278 (Mouse)

Gene Symbol

BCAM

UniProt

CAA58449.1

Additional BCAM Products

Product Documents for Recombinant Human BCAM His-tag Protein, CF

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Product Specific Notices for Recombinant Human BCAM His-tag Protein, CF

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