Recombinant Human Bcl-w (minus C-Terminus) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 824-BW

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

Q92843

Structure / Form

Monomer and disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Bcl-w Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Bcl-w protein
Ala2-Thr172, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

19.5 kDa

SDS-PAGE

18 kDa, under reducing and non-reducing conditions

Activity

Measured by its ability to inhibit Caspase-8-cleaved BID-mediated release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0) to quantify cytochrome c.
The typical EC50 is between 50 and 250 nM in the presence of 54 nM Recombinant Human BID Caspase-8-cleaved (Catalog # 882-B8).
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

824-BW
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Bcl-w

Bcl-w (also known as Bcl-2 like protein 2, BCL2L2) is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
  3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
  4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
  5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.

Long Name

B Cell Lymphoma/Leukemia w

Alternate Names

BCL2L2, Bclw

Entrez Gene IDs

599 (Human); 12050 (Mouse)

Gene Symbol

BCL2L2

UniProt

Q92843

Additional Bcl-w Products

Product Documents for Recombinant Human Bcl-w (minus C-Terminus) Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Bcl-w (minus C-Terminus) Protein, CF

For research use only

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