Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6454-BR

R&D Systems, part of Bio-Techne
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6454-BR-020
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Key Product Details

Source

E. coli

Accession #

P53004

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Biliverdin Reductase A/BLVRA Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Biliverdin Reductase A/BLVRA protein
Glu6-Ser294, with an N-terminal Met and C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

34 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by the reduction of biliverdin IX alpha to bilirubin.
The specific activity is >450 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6454-BR
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Biliverdin Reductase A/BLVRA

The clearance of heme in mammals is a two-step process starting with the conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by biliveredin reductase. Biliverdin Reductase A (BLVRA) converts the alpha isomer of biliverdin IX, which constitutes 95‑97% of total biliverdin isomers in adults, to bilirubin IX-alpha (1). Although bilirubin is known to be a toxic pigment that needs to be excreted, it is also a physiological antioxidant (2). Therefore BLVRA enables continuous protection of cells against oxidative stress. BLVRA is a cytosolic protein that is highly expressed in the liver, but is also present in most tissues. The two N‑terminal residues are a pro-sequence that is missing in the mature protein (3). The reduction of biliverdin by BLVRA is coupled to oxidation of pyridine nucleotide co‑factors NADH and NADPH with distinct pH optima, 6.7 for NADH and 8.7 for NADPH (4). BLVRA has many functions independent of its reductase activity. It is also a dual-specificity (Ser/Thr + Tyr) protein kinase (5) and an activator of protein kinase C bII (6) and ERK (7). It functions as a transcription factor regulating heme oxygenase gene expression (8).

References

  1. Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343.
  2. Stocker, R. et al. (1987) Science 235:1043.
  3. Fakhrai, H. and M.D. Maines. (1992) J. Biol. Chem. 267:4023.
  4. Maines, M. D. et al. (1996) Eur. J. Biochem. 235:372.
  5. Kapitulnik, J. and M.D. Maines. (2009) Trends Pharmacol. Sci. 30:129.
  6. Maines, M.D. et al. (2007) J. Biol. Chem. 282:8110.
  7. Lerner-Marmarosh, N. et al. (2008) Proc. Natl. Acad. Sci. USA. 105:6870.
  8. Tudor, C. et al. (2008) Biochem. J. 413:405.

Alternate Names

BLVRA, BVR, BVRA

Entrez Gene IDs

644 (Human)

Gene Symbol

BLVRA

UniProt

P53004

Additional Biliverdin Reductase A/BLVRA Products

Product Documents for Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

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