Recombinant Human Blood Group B Transferase/GTB Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6824-GT

R&D Systems, part of Bio-Techne
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6824-GT-020
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Key Product Details

Source

CHO

Accession #

AAD26574

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Blood Group B Transferase/GTB Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Blood Group B Transferase/GTB protein
Arg63-Pro354, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

34.8 kDa

SDS-PAGE

35-40 kDa, reducing conditions

Activity

Measured by its ability to transfer galactose from UDP-galactose to lacto-N-fucopentaose.
The specific activity is >1,250 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6824-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Blood Group B Transferase/GTB

The ABO blood group type is characterized by the different carbohydrate antigens displayed on the cell surface of human erythrocytes. Blood group A exhibits the A antigen, GalNAc alpha1-3(Fuc alpha1-2)Gal. Blood group B exhibits the B antigen, Gal alpha1-3(Fuc alpha1-2)Gal. Blood group O exhibits the H antigen, Fuc alpha1-2Gal. The H antigen is converted to either the A antigen via GTA, an alpha1-3 N‑acetylgalactosaminyltransferase, or to the B antigen via GTB, an alpha1-3 galactosyltransferase. GTA and GTB are encoded by different alleles of the ABO gene and differ at only four amino acids (R176G, G235S, L266M, and G268A). People with type O blood do not have a functional transferase expressed due to a frameshift mutation in the ABO gene. Both GTA and GTB are type II Golgi-resident transmembrane proteins but can also be found in secreted forms in body fluids (1, 2, 3, 4, 5). The activity of the recombinant human GTB was determined using a phosphatase-coupled glycosyltransferase assay (6).

References

  1. Marcus, S.L. et al. (2003) J. Biol. Chem. 278:12403.
  2. Persson, M. et al. (2007) J. Biol. Chem. 282:9564.
  3. Yamamoto, F. et al. (1990) J. Biol. Chem. 265:19257.
  4. Yamamoto, F. et al. (1990) Nature 345:229.
  5. Patenaude, S.I. et al. (2002) Nat. Struct. Biol. 9:685.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

Fucosylglycoprotein 3-alpha-galactosyltransferase

Alternate Names

A3GALT1, ABO

Entrez Gene IDs

28 (Human); 80908 (Mouse); 65270 (Rat)

Gene Symbol

ABO

UniProt

AAD26574

Additional Blood Group B Transferase/GTB Products

Product Documents for Recombinant Human Blood Group B Transferase/GTB Protein, CF

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Product Specific Notices for Recombinant Human Blood Group B Transferase/GTB Protein, CF

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