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Recombinant Human BMP-4/BMP-7 Heterodimer Protein

R&D Systems, part of Bio-Techne | Catalog # 3727-BP

R&D Systems, part of Bio-Techne
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Carrier Free
3727-BP-010/CF

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With Carrier
3727-BP-010

Key Product Details

Source

E. coli

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human BMP-4/BMP-7 Heterodimer protein
Met Human BMP-4
(Ser293-Arg408)
Accession # P12644
Human BMP-7
(Ser293-His431)
Accession # P18075
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met (BMP-4) & Ser293 (BMP-7)

Predicted Molecular Mass

13.3 kDa (BMP-4), 15.8 kDa (BMP-7)

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 15-75 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3727-BP
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 3727-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4/BMP-7 Heterodimer

Human Bone Morphogenetic Protein 4 and 7 (BMP-4 and BMP-7), also known as osteogenic protein 1 (OP-1), belong to distinct BMP subgroups of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-4 and BMP-7 are widely expressed from early embryogenesis to adult and influence a variety of morphogenic processes (1-3). The human BMP-4 cDNA encodes a 408 amino acid (aa) precursor that includes a 19 aa signal sequence, a 273 aa propeptide, and a 116 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that includes a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed intracellulaly by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Cell types that express both BMP components can produce the heterodimers. BMP-4 and BMP-7 are each 98% conserved between human and mouse. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60 - 70% aa sequence identity with BMP-5, -6, and -8 and less than 50% aa sequence identity with other BMPs. BMP‑4 and BMP‑7, when associated into 36 kDa heterodimers (6, 7), acquire increased potency in in vitro osteogenic differentiation assays and in vivo bone formation models compared to either homodimer (6-10). Furthermore, BMP-4/BMP-7 heterodimers induce ventral mesoderm and blood formation in mid-blastula Xenopus animal cap assays, whereas the homodimers do not (11, 12). BMP-4/BMP-7 as well as either homodimer can modulate mesoderm induction by activin and prevent subsequent dorsalization of the mesoderm (12).

References

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Oida, S. et al. (1995) DNA Seq. 5:273.
  5. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  6. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  7. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859.
  8. Kusumoto, K. et al. (1997) Biochem. Biophys. Res. Commun. 239:575.
  9. Zhao, M. et al. (2005) J. Cell Biochem. 95:1.
  10. Israel, D.I. et al. (1996) Growth Factors 13:291.
  11. Suzuki, A. et al. (1997) Biochem. Biophys. Res. Commun. 232:153.
  12. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.

Long Name

Bone Morphogenetic Protein 4/7

Entrez Gene IDs

652 (Human)

Gene Symbol

BMP4

Additional BMP-4/BMP-7 Heterodimer Products

Product Documents for Recombinant Human BMP-4/BMP-7 Heterodimer Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BMP-4/BMP-7 Heterodimer Protein

For research use only

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