Recombinant Human BMP-8b Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9316-BP
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-8b protein
Ala264-His402
Ala264-His402
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala264
Predicted Molecular Mass
16 kDa
SDS-PAGE
17-20 kDa, reducing conditions
Activity
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326.
The ED50 for this effect is 0.7-3.5 μg/mL.
The ED50 for this effect is 0.7-3.5 μg/mL.
Formulation, Preparation and Storage
9316-BP
Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
Reconstitution |
Reconstitute at 250 μg/mL in 4 mM HCl.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BMP-8b
References
- Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
- Chen, D. et al. (2004) Growth Factors 22:233.
- Bragdon, B. et al. (2010) Cell Signal. Oct 16 epub.
- Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
- Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
- Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
- Zhao, G.-Q. et al. (1998) Development 125:1103.
- Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
- Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
- van der Horst, G. et al. (2002) Bone 31:661.
- Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
- Paic, F. et al. (2009) Bone 45:682.
- Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
- Cheng, Z. et al. (2014) Oncol. Rep. 32:1861.
- Whittle, A.J. et al. (2012) Cell 149:871.
- Martins, L. et al. (2016) Cell Rep. 16:2231.
Long Name
Bone Morphogenetic Protein 8b
Alternate Names
OP-3
Gene Symbol
BMP8B
UniProt
Additional BMP-8b Products
Product Documents for Recombinant Human BMP-8b Protein, CF
Product Specific Notices for Recombinant Human BMP-8b Protein, CF
For research use only
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