Recombinant Human Brevican Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4009-BC

R&D Systems, part of Bio-Techne
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4009-BC-050
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Key Product Details

Source

NS0

Accession #

AAH09117

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Binding Activity

View all Brevican Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Brevican protein
Asp23-Pro911, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23

Predicted Molecular Mass

97.7 kDa

SDS-PAGE

130-160 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated hyaluronan in a functional ELISA with an estimated Kd <3 nM.

Formulation, Preparation and Storage

4009-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution
Reconstitute at 200 μg/mL in sterile deionized water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brevican

Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig-like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3-7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is up-regulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is up-regulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N-terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig-like and two link domains, brevican shares 45%-51% aa sequence identity with aggrecan, neurocan, and versican.

References

  1. Viapiano, M.S. and R.T. Matthews (2006) Trends Mol. Med. 12:488. 
  2. Gary, S.C. et al. (2000) Gene 256:139. 
  3. Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495. 
  4. Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206. 
  5. Hamel, M.G. et al. (2005) J. Neurochem. 93:1533. 
  6. Ogawa, T. et al. (2001) J. Comp. Neurol. 432:285  
  7. Yamada, H. et al. (1997) J. Neurosci. 17:7784.  
  8. Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
  9. Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
  10. Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
  11. Mayer, J. et al. (2005) BMC Neurosci. 6:52.
  12. Yuan, W. et al. (2002) Neuroscience 114:1091.
  13. Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
  14. Jaworski, D.M. et al. (1999) Exp. Neurol. 157:327.
  15. Viapiano, M.S. et al. (2005) Cancer Res. 65:6726.
  16. Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.

Long Name

Brain Specific Proteoglycan in the Aggrecan Family

Alternate Names

ALPBRE, BCAN, BEHAB, CSPG7

Entrez Gene IDs

63827 (Human); 12032 (Mouse); 25393 (Rat)

Gene Symbol

BCAN

UniProt

AAH09117

Additional Brevican Products

Product Documents for Recombinant Human Brevican Protein, CF

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Product Specific Notices for Recombinant Human Brevican Protein, CF

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