Recombinant Human BTNL9 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9659-BT

R&D Systems, part of Bio-Techne
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9659-BT-050
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Key Product Details

Source

HEK293

Accession #

Q6UXG8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BTNL9 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTNL9 protein
Human BTNL9
(Ser35-Lys256)
Accession # Q6UXG8-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser35

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-69 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 fot this effect is 3-15 μg/mL.

Scientific Data Images for Recombinant Human BTNL9 Fc Chimera Protein, CF

Recombinant Human BTNL9 Fc Chimera Protein Bioactivity

Recombinant Human BTNL9 Fc Chimera Protein Bioactivity

Recombinant Human BTNL9 Fc Chimera (Catalog # 9659-BT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 3-15 μg/mL.

Formulation, Preparation and Storage

9659-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL9

Butyrophilin-like 9 (BTNL9) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL9 is a 535 amino acid (aa) type I transmembrane glycoprotein that contains a 222 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 258 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in the BTN/BTNL proteins, but the IgC domain is relatively weak (1-4). The ECD of human BTNL9 shares 66% and 67% aa sequence identity with rat and mouse, respectively. Human BTNL9 mRNA has been identified in adipose, lung, thymus, spleen, colon, and cardiac tissues, but its highest levels of expression were found in B cells (2, 5). Although the specific function of BTNL9 has yet to be fully elucidated, recombinant BTNL9-Fc has been shown to bind to many immune cells including T cells, B cell, macrophages, and dendritic cells (6). BTNL9 expression has also been found to be down-regulated in colon cancer tumors (5). R&D in house data indicate that BTNL9 protein inhibits the secretion of IFN-gamma from anti-CD3 activated PBMC.

References

  1. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  4. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
  5. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.
  6. Yamazaki, T. et al. (2010) J. Immunol. 185:5907.

Long Name

Butyrophilin-like 9

Alternate Names

BTN3, VDLS1900

Entrez Gene IDs

153579 (Human); 237754 (Mouse); 686977 (Rat)

Gene Symbol

BTNL9

UniProt

Q6UXG8

Additional BTNL9 Products

Product Documents for Recombinant Human BTNL9 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human BTNL9 Fc Chimera Protein, CF

For research use only

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