Recombinant Human C1qR1/CD93 His-tag Protein, CF

Name: Recombinant Human C1qR1/CD93 His-tag Protein, CF
Brand: R&D Systems
SKU: 11587-CD

R&D Systems, part of Bio-Techne | Catalog # 11587-CD

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Product Datasheet / COA / SDS

Key Product Details

Source:	CHO
Accession #:	Q9NPY3.3
Applications:	Bioactivity

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Technical Data
Citations / Reviews

Product Specifications for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human C1qR1/CD93 protein Val258-Lys580 with a C-terminal 6-His tag
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val258
Predicted Molecular Mass	34 kDa
SDS-PAGE	34 kDa
Activity	Measured by its binding ability in a functional ELISA. Recombinant Human C1qR1/CD93 His-tag binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (Catalog # 1334-B7) with an ED₅₀ of 0.150-1.50 μg/mL.

Scientific Data Examples for Recombinant Human C1qR1/CD93 His-tag Protein, CF

	Recombinant Human C1qR1/CD93 His-tag Protein Binding Activity. Measured by its binding ability in a functional ELISA. Recombinant Human C1qR1/CD93 His-tag Protein binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (1334-B7) with an ED₅₀ of 0.150-1.50 μg/mL.
	Recombinant Human C1qR1/CD93 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human C1qR1/CD93 His-tag Protein (Catalog # 11587-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11587-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in sterile water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1qR1/CD93

C1qR1, also known as CD93 and C1qRp, is an approximately 125 kDa type-1 transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) containing C‑type lectin and EGF‑like domains, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). CD93 plays a role in cardiovascular disease progression and modulates angiogenesis, inflammation and tumor growth and its interaction with insulin-like growth factor binding protein 7 (IGFBP7) contributes to abnormal tumor vasculature (16-17).

Item	Value
References	Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411. Nepomuceno, R.R. et al. (1997) Immunity 6:119. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929. McGreal, E.P. et al. (2002) J. Immunol. 168:5222. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353. Jeon, J.W. et al. (2010) J. Immunol. 185:4921. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583. Tossetta, G. et al. (2023) Cells 12:1778. Sun, Y. et al. (2021) Sci. Transl. Med. 13:eabc8922.
Long Name	Complement Component C1q Receptor
Entrez Gene IDs	22918 (Human); 17064 (Mouse); 84398 (Rat)
Alternate Names	AA4 Antigen, C1q R1, C1q Rp, C1q receptor 1, C1q/MBL/SPA receptor, C1qR(p), C1qR1, C1qRp, CD93, CD93 antigenC1qR, CD93 molecule, CDw93C1QR1, Collectin Receptor, Complement component 1 q subcomponent receptor 1, ECSM3, Ly68, MXRA4, Matrix-remodeling-associated protein 4, complement component 1, q subcomponent, receptor 1, complement component C1q receptor, dJ737E23.1, matrix-remodelling associated 4

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Recombinant Human C1qR1/CD93 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11587-CD

Key Product Details

Product Specifications for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Scientific Data Examples for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: C1qR1/CD93

Customer Reviews for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Product Documents for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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