Recombinant Human C1qR1/CD93 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11587-CD

R&D Systems, part of Bio-Techne
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11587-CD-050
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Key Product Details

Source

CHO

Accession #

Q9NPY3.3

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qR1/CD93 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human C1qR1/CD93 protein
Val258-Lys580 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val258

Predicted Molecular Mass

34 kDa

SDS-PAGE

65-80 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human C1qR1/CD93 His-tag binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (Catalog # 1334-B7) with an ED50 of 0.150-1.50 μg/mL.

Scientific Data Images for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Recombinant Human C1qR1/CD93 His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human C1qR1/CD93 His-tag Protein binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (1334-B7) with an ED50 of 0.150-1.50 μg/mL.

Recombinant Human C1qR1/CD93 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human C1qR1/CD93 His-tag Protein (Catalog # 11587-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa, under reducing conditions.

Formulation, Preparation and Storage

11587-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qR1/CD93

C1qR1, also known as CD93 and C1qRp, is an approximately 125 kDa type-1 transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) containing C‑type lectin and EGF‑like domains, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). CD93 plays a role in cardiovascular disease progression and modulates angiogenesis, inflammation and tumor growth and its interaction with insulin-like growth factor binding protein 7 (IGFBP7) contributes to abnormal tumor vasculature (16-17).

References

  1. Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
  2. Nepomuceno, R.R. et al. (1997) Immunity 6:119.
  3. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
  4. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
  5. McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
  6. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
  7. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
  8. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
  9. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
  10. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
  11. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
  12. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
  13. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
  14. Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
  15. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.
  16. Tossetta, G. et al. (2023) Cells 12:1778.
  17. Sun, Y. et al. (2021) Sci. Transl. Med. 13:eabc8922.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

Q9NPY3.3

Additional C1qR1/CD93 Products

Product Documents for Recombinant Human C1qR1/CD93 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human C1qR1/CD93 His-tag Protein, CF

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