Recombinant Human Cadherin-10 (CAD-10) Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 6770-CA

R&D Systems, part of Bio-Techne
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6770-CA-050
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Key Product Details

Source

CHO

Accession #

Q9Y6N8

Conjugate

Unconjugated

Applications

Bioactivity

View all Cadherin-10 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cadherin-10 protein
Human Cadherin-10
(Glu23-Ala613)
Accession # Q9Y6N8		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23 & Gly55

Predicted Molecular Mass

92.7 kDa (monomer)

SDS-PAGE

100-120 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 0.1-0.4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

6770-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-10

Cadherin-10, also known as T2-Cadherin, is an approximately 120 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human Cadherin-10 is synthesized with a 22 amino acid (aa) signal peptide and a 32 aa N‑terminal propeptide. The mature cell surface-expressed protein consists of a 559 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3, 4). Within the propeptide and ECD, human Cadherin-10 shares 97% aa sequence identity with mouse and rat Cadherin-10. It interacts homotypically as well as hetertypically with Cadherin-9 and more weakly with Cadherin-6 (4). Cadherin-10 is expressed in the central nervous system, particularly in the limbic system, frontal cortex, somatosensory barrel cortex (in rat), olfactory structures, epithelial cells lining the choroid plexus and ventricles, retinal pigmented epithelium, and endothelial cells of the brain and retinal microvasculature (3, 5‑10). It is also expressed on epithelial luminal cells of the prostate, as well as in testes, ovary, placenta, kidney, small intestine, and on CD4+CD8+ T cells (3, 10‑13). Cadherin-10 expression is decreased postnatally in the barrel cortex and testes (8, 12). It is also down‑regulated in glioblastoma vasculature and prostate cancer (10, 11). Polymorphisms of human Cadherin-10 are associated with autism spectrum disorders (6).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Kools, P. et al. (1999) FEBS Lett. 452:328.
  4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  5. Fushimi, D. et al. (1997) Dev. Dyn. 209:269.
  6. Wang, K. et al. (2009) Nature 459:528.
  7. Bekirov, I.H. et al. (2002) Neuroscience 115:213.
  8. Gil, O.D. et al. (2002) J. Comp. Neurol. 453:372.
  9. Akins, M.R. et al. (2007) J. Comp. Neurol. 501:483.
  10. Williams, M.J. et al. (2005) Brain Res. 1058:62.
  11. Walker, M.M. et al. (2008) Mod. Pathol. 21:85.
  12. Munro, S.B. and O.W. Blaschuk (1996) Biol. Reprod. 55:822.
  13. Munro, S.B. et al. (1996) Cell. Immunol. 169:309.

Alternate Names

CAD10, Cadherin10, CDH10, T2-Cadherin

Entrez Gene IDs

1008 (Human); 320873 (Mouse); 29181 (Rat)

Gene Symbol

CDH10

UniProt

Q9Y6N8

Additional Cadherin-10 Products

Product Documents for Recombinant Human Cadherin-10 (CAD-10) Fc Chimera, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cadherin-10 (CAD-10) Fc Chimera, CF

For research use only

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