Recombinant Human Calsyntenin-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5346-CL

R&D Systems, part of Bio-Techne
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5346-CL-050
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Key Product Details

Source

NS0

Accession #

AAH33902

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Calsyntenin-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Calsyntenin-1 protein
Arg30-Thr851, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg30

Predicted Molecular Mass

92.8 kDa

SDS-PAGE

110-120 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
The ED50 for this effect is typically 1-4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5346-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calsyntenin-1

Calsyntenin-1 (CST-1; gene name CLSTN1), also called Alcadein-alpha, is a 140‑150 kDa type I transmembrane glycoprotein that is member of the Alcadein family of cadherin domain-containing molecules (1, 2). The human Calsyntenin-1 cDNA encodes 981 amino acids (aa), including a 28 aa signal sequence and the 953 aa mature protein (1). Further cleavage by ADAM10 and ADAM17 generates an N-terminal 115 kDa portion containing the two cadherin domains (aa 38‑265), termed soluble Alc‑ alpha, and a C-terminal portion, termed CTF1-alpha (1, 3). CTF1-alpha contains 34 extracellular aa, 21 transmembrane aa, and 101 cytoplasmic aa that are highly acidic and bind calcium (1, 4). Isoforms of 962-994 aa contain a 78 aa substitution for aa 917‑981, with or without deletions of aa 72‑81 and 507‑525. Within the soluble Alc‑ alpha portion (aa 30‑851), human Calsyntenin-1 shares 92% aa sequence identity with mouse, 93% with equine, and 91% with rat, canine, bovine and porcine Calsyntentin-1. It is expressed in neuronal post-synaptic plasma membranes, neuronal APP-containing early endosomal vesicles (full-length CST-1) and APP-negative recycling‑endosomal vesicles (CST-1 cleaved form), plus the secretory granules of anterior pituitary basophilic gonadotrophs and pancreatic islet alpha-cells (1-6). Soluble Alc‑ alpha mediates adhesion and can be released from axons, while CTF1-alpha can be internalized and mediates trafficking of vesicles via interaction with Kinesin-1 (1, 6-9). This interaction facilitates intracellular transport of vesicles along microtubules in axonal growth cones (7-10). Calsyntenin regulates amyloid precursor protein (APP) cleavage and transport, and perturbation of axonal transport of APP by CST-1 containing carriers results in increased production of A beta. Finally, CST-1 may also play a regulatory role in post-synaptic signaling (1, 2, 4, 8, 10).

References

  1. Vogt, L. et al. (2001) Mol. Cell. Neurosci. 17:151.
  2. Araki, Y. et al. (2003) J. Biol. Chem. 278:49448.
  3. Hata, S. et al. (2009) J. Biol. Chem. 284:36024.
  4. Hintsch, G. et al. (2002) Mol. Cell. Neurosci. 21:393.
  5. Rindler, M.J. et al. (2008) J. HIstochem. Cytochem. 56:381.
  6. Steuble, M. et al. (2010) Proteomics 10:3775.
  7. Konecna, A. et al. (2006) Mol. Biol. Cell 17:3651.
  8. Araki, Y. et al. (2007) EMBO J. 26:1475.
  9. Vagnoni, A. et al. (2011) J. Cell Sci. 24:1032.
  10. Ludwig, A. et al. (2009) Traffic 10:572.
  11. Vagnoni, A. et al. (2012) Hum. Mol. Genet. Mar. 27 [Epub ahead of print].

Alternate Names

Alcadein-alpha, Calsyntenin1, CLSTN1, CS1, CSTN1, XB31alpha

Entrez Gene IDs

22883 (Human); 65945 (Mouse); 313717 (Rat)

Gene Symbol

CLSTN1

UniProt

AAH33902

Additional Calsyntenin-1 Products

Product Documents for Recombinant Human Calsyntenin-1 Protein, CF

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Product Specific Notices for Recombinant Human Calsyntenin-1 Protein, CF

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