Recombinant Human Carbonic Anhydrase III Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2185-CA
Key Product Details
Product Specifications
Source
E. coli-derived human Carbonic Anhydrase III/CA3 protein
Ala2-Lys260
Ala2-Lys260
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala2
Predicted Molecular Mass
31 kDa
SDS-PAGE
30 kDa, reducing conditions
Activity
Measured by its esterase activity.
The specific activity is >1 pmol/min/µg, as measured under the described conditions.
The specific activity is >1 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2185-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 1 mg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carbonic Anhydrase III/CA3
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).
Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth (2).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
Alternate Names
CA3
Entrez Gene IDs
761 (Human)
Gene Symbol
CA3
UniProt
Additional Carbonic Anhydrase III/CA3 Products
Product Documents for Recombinant Human Carbonic Anhydrase III Protein, CF
Product Specific Notices for Recombinant Human Carbonic Anhydrase III Protein, CF
For research use only
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