Recombinant Human Carboxypeptidase B1/CPB1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2897-ZN
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Carboxypeptidase B1/CPB1 protein
His16-Tyr417, with a C-terminal 10-His tag
His16-Tyr417, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His16
Predicted Molecular Mass
47 kDa
SDS-PAGE
45 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg.
The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2897-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carboxypeptidase B1/CPB1
Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis (2). The deduced amino acid sequence of human CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 110), and a mature chain (residues 111 to 417). The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro (1).
References
- Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 831, Academic Press, San Diego.
- Yamamoto, K.K. et al. (1992) J. Biol. Chem. 267:2575.
Alternate Names
CPB1, PASP, PCPB
Gene Symbol
CPB1
UniProt
Additional Carboxypeptidase B1/CPB1 Products
Product Documents for Recombinant Human Carboxypeptidase B1/CPB1 Protein, CF
Product Specific Notices for Recombinant Human Carboxypeptidase B1/CPB1 Protein, CF
For research use only
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