Recombinant Human Caspr1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2418-CR

R&D Systems, part of Bio-Techne
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2418-CR-050
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Key Product Details

Source

CHO

Accession #

P78357

Conjugate

Unconjugated

Applications

Bioactivity

View all Caspr1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Caspr1 protein
Trp20-Gly1280, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp20

Predicted Molecular Mass

144 kDa

SDS-PAGE

130-165 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human APP (Catalog # 3466-PI) is immobilized at 4 µg/mL (100 µL/well), Recombinant Human Caspr1 binds with an ED50 of 2-12 μg/mL.

Scientific Data Images for Recombinant Human Caspr1 His-tag Protein, CF

Recombinant Human Caspr1 His-tag Protein SDS-PAGE

Recombinant Human Caspr1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Caspr1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-165 kDa.

Formulation, Preparation and Storage

2418-CR
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Caspr1

Caspr1 (contactin-associated protein 1) is a 190 kDa neuronal transmembrane protein that belongs to the neurexin superfamily. It consists of a large extracellular domain (ECD), a single transmembrane region, and a short intracellular region. The mature ECD region contains a factor VII/discoidin region, a laminin G domain, and a fibrinogen domain along with additional laminin G and epidermal growth factor domains. The intracellular domain includes a proline-rich sequence capable of binding to a subclass of SH3 domains of specific molecular signals, including Src and Fyn tyrosine kinases (1). The mature ECD of human Caspr1 shares 94% amino acid identity with both the mouse and rat proteins. Caspr1 is located at the paranodes of the myelinated axons and plays an important role in the formation and stability of this structure. It forms a complex with contactin and NFASC-155 (NF-155), acts as a barrier between the nodes of Ranvier and internodes, and is involved in the propagation of action potentials and mediation of signal transport (2). It may mediate the timing of neuron and astrocyte differentiation in neural progenitor cells (3). Caspr1 may form a g-secretase associated complex, is distributed around amyloid plaques in the cerebral cortex of mice interacting with amyloid precursor proteins, thereby potentially be involved in the pathology of Alzheimer's disease (4, 5).

References

  1. Poliak, S. et al. (1999) Neuron 24:1037.
  2. Sherman, D. L. et al. (2005) Neuron 48:737.
  3. Wu, Z. Q. et al. (2017) Cereb. Cortex. 27:1369.
  4. Hur, J.Y. et al. (2012) J. Biol. Chem. 287:11991.
  5. Zou, Y. et al. (2017) Neural Regen. Res. 12:1551.

Long Name

Contactin Associated Protein-like 1

Alternate Names

CNTNAP, CNTNAP1, p190

Entrez Gene IDs

8506 (Human); 53321 (Mouse)

Gene Symbol

CNTNAP1

UniProt

P78357 (Human)

Additional Caspr1 Products

Product Documents for Recombinant Human Caspr1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human Caspr1 His-tag Protein, CF

For research use only

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