Recombinant Human Cathepsin A/Lysosom Carboxypeptidase A, CF
R&D Systems, part of Bio-Techne | Catalog # 1049-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin A/Lysosomal Carboxypeptidase A protein
Ala29-Tyr480, with a C-terminal 10-His tag
Ala29-Tyr480, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala29
Predicted Molecular Mass
53 kDa
SDS-PAGE
51-62 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >75 pmol/min/µg, as measured under the described conditions.
The specific activity is >75 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1049-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin A/Lysosomal Carboxypeptidase A
Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta-galactosidase ( beta-gal) and neuraminidase is essential for beta-gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta-gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.
References
- Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego.
- Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.
Alternate Names
CTSA, Lysosomal Carboxypeptidase A
Gene Symbol
CTSA
UniProt
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Product Documents for Recombinant Human Cathepsin A/Lysosom Carboxypeptidase A, CF
Product Specific Notices for Recombinant Human Cathepsin A/Lysosom Carboxypeptidase A, CF
For research use only
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