Recombinant Human CCL13/MCP-4 Protein
R&D Systems, part of Bio-Techne | Catalog # 327-P4
Key Product Details
Product Specifications
Source
E. coli-derived human CCL13/MCP-4 protein
Gln24-Thr98
Gln24-Thr98
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln24
Predicted Molecular Mass
8.6 kDa
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485.
The ED50 for this effect is 0.02-0.06 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 0.5-2 µg/mL.
The ED50 for this effect is 0.02-0.06 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 0.5-2 µg/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 327-P4
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 327-P4/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL13/MCP-4
Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65 - 66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.
References
- Garcia-Zepeda, E.A. et al. (1996) J. Immunol. 157:5613.
- Uguccioni, M. et al. (1996) J. Exp. Med. 183:2379.
- Godisha, R. et al. (1997) J. Leukoc, Biol. 61:353.
- Stellato, C. et al. (1997) J. Clin. Invest. 99:926.
- Heath, H. et al. (1997) J. Clin. Invest. 99:178.
- Charo, I.F. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2752.
- Daugherty, B.L. et al. (1996) J. Exp. Med. 183:2349.
- Combadiere, C. et al. (1995) J. Biol. Chem. 270:16491.
Alternate Names
MCP-4, MCP4
Entrez Gene IDs
6357 (Human)
Gene Symbol
CCL13
UniProt
Additional CCL13/MCP-4 Products
Product Documents for Recombinant Human CCL13/MCP-4 Protein
Product Specific Notices for Recombinant Human CCL13/MCP-4 Protein
For research use only
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