Recombinant Human CD151 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1884-CD

R&D Systems, part of Bio-Techne
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1884-CD-050
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Key Product Details

Source

CHO

Accession #

P48509

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD151 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD151 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human CD151-LEL
(Ala113-Arg221)
Accession # P48509
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

39 kDa

SDS-PAGE

41-55, and 90-102 kDa (non-reducible dimer), reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Integrin  alphaV beta3 (Catalog # 3050-AV) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD151 Fc Chimera binds with an ED50 of 0.3-1.8 μg/mL.  

Scientific Data Images for Recombinant Human CD151 Fc Chimera Protein, CF

Recombinant Human CD151 Fc Chimera Protein Binding Activity

Recombinant Human CD151 Fc Chimera Protein Binding Activity

When Recombinant Human Integrin aV beta3 (Catalog # 3050-AV) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD151 Fc Chimera binds with an ED50 of 0.3-1.8 µg/mL.
Recombinant Human CD151 Fc Chimera Protein SDS-PAGE

Recombinant Human CD151 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD11b/Integrin alpha M was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-55 kDa and 80 - 110 kDa, respectively.

Formulation, Preparation and Storage

1884-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD151

Human CD151, also known as SFA-1, Tetraspanin-24, and GP27, is a palmitoylated glycoprotein in the tetraspanin superfamily. It is the first tetraspanin member to be identified as a promoter of cancer metastasis (1, 2), and it is found to participate in nearly all stages of cancer progression (3). CD-151 is normally expressed in endothelial cells, platelets, and frequently over-expressed in cancer cells (4). Mature CD-151 is a multi-pass membrane protein that contains four transmembrane domains, three cytoplasmic domains, and two extracellular loops. The region of amino acids (aa) 113-221 contains the largest extracellular loop (LEL) that involves in interacting with integrins and regulating integrin functions (5). Human CD151 LEL shares 88.9% and 87.0% aa identity with that of mouse and rat respectively. CD151 interacts with integrins such as alphav beta3, alpha3 beta1, alpha6 beta1, alpha7 beta1, and alpha6 beta4 to regulate their activities and thus resulting in modulation of adhesion, spreading, migration, angiogenesis, invasion and metastasis (1, 3-5). CD151 can also complex with immunoglobulin super family proteins and other tetraspanins such as CD9, CD81, and CD63 (3). Clinically, high levels of CD151 are correlated with poor prognosis in a variety of tumors (3, 4). CD151 has been implicated as a potential diagnostic marker in osteosarcoma and prostate cancer and a putative target for antibody-based immunotherapy (3). CD-151 is a key player in the formation of basement membranes in kidney and skin tissues; it is also associated with human papillomavirus (HPV) infection (6, 7).

References

  1. Detchokul, S. et al. (2014) Br. J. Pharmacol. 171(24):5462.
  2. Hasegawa, H. et al. (1996). Journal of Virology 70: 3258.
  3. Sadej, R. et al. (2014) Laboratory Investigation 94:41.
  4. Kumari, S. et al. (2015) Biomark Cancer 7:7.
  5. Yu J. et al. (2017) Biochem. J. 474(4):589.
  6. Scheffer, K.D. et al. (2014) Viruses 6:893.
  7. Karamatic Crew, V. et al. (2004) Blood 104:2217.

Alternate Names

CD151, PETA-3, SFA-1, TSPAN24

Entrez Gene IDs

977 (Human); 12476 (Mouse); 64315 (Rat)

Gene Symbol

CD151

UniProt

P48509

Additional CD151 Products

Product Documents for Recombinant Human CD151 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human CD151 Fc Chimera Protein, CF

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