Recombinant Human CD164 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9679-CD

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q04900-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

View all CD164 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD164 protein
Human CD164
(Asp24-Thr160)
Accession # Q04900-1		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp24

Predicted Molecular Mass

41 kDa

SDS-PAGE

103-115 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9679-CD
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD164

CD164 is a mucin-like glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells. It is composed of a signal sequence, an extracellular domain, a transmembrane domain and an intracellular domain (1). The CD164 cDNA encodes a 178-amino acid polypeptide core, of which residues 140‑164 represent the predicted transmembrane domain and residues 165-178 form a short cytoplasmic domain (2). Biochemical and functional analyses of human hematopoietic cell lines and transfectants demonstrate that CD164 comprises a homodimeric molecule of approximately 160 kDa. It plays an important role in mediating or regulating hematopoietic precursor cell adhesion to stroma, and may serve as a potent negative regulator of hematopoietic progenitor cell proliferation (3). Moreover, recent studies have indicated that CD164 may be a basophil activation marker, potentially useful in diagnosis of allergy caused by variety of allergens, including drug allergen (4). The molecule is extensively glycosylated with sites of O-linked glycosylation clustered in two discrete mucin domains separated by a more globular cysteine-rich region (1).

References

  1. Zannettino, A.C. et al. (1998) Blood 92:2613.
  2. Watt, S.M. et al. (1998) Blood 92:849.
  3. Watt, S.M. and J.Y. Chan (2000) Leuk. Lyphoma. 37:1.
  4. Wolanczyk-Medrala, A. et al. (2011) Curr. Pharm. Des. 17:3786.

Alternate Names

CD164, MGC-24, MUC-24

Entrez Gene IDs

8763 (Human); 53599 (Mouse)

Gene Symbol

CD164

UniProt

Q04900-1

Additional CD164 Products

Product Documents for Recombinant Human CD164 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD164 Fc Chimera Protein, CF

For research use only

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