Recombinant Human CD300c Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3256-LM
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CD300c protein
| Human CD300c (Met29-Arg183) Accession # Q08708 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met29
Predicted Molecular Mass
43.5 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and Recombinant Human CD300c Fc Chimera.
The ED50 for this effect is 4-16 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and Recombinant Human CD300c Fc Chimera.
The ED50 for this effect is 4-16 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
3256-LM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD300c
Leukocyte mono-Ig-like receptor 2 (LMIR2; also CMRF-35, CMRF35-A antigen, and CD300c antigen) is a 23 kDa (predicted) type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of molecules within the immunoglobulin (Ig) superfamily (1-4). Human LMIR2 is synthesized as a 224 amino acid (aa) precursor that has a 20 aa signal sequence, a 163 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 20 aa cytoplasmic tail (SwissProt # Q08708). The ECD contains an Ig-like V-type domain (aa’s 22-128) and two N-linked glycosylation sites (aa’s 90 and 99). Downstream of the Ig V-domain, the membrane proximal region of LMIR2 (aa 128-183) contains a high proportion of proline (18%), serine (20%) and threonine (13%) residues (1). The transmembrane segment contains a charged glutamic acid that contributes to cell activation (1-3). Human LMIR2 shares 52% aa sequence identity with the mouse LMIR2, which is also known as CLM4. Human LMIR2 is 90% identical to human LMIR1 within the Ig-like domain. LMIR2 is present on the surface of natural killer cells, granulocytes, most myeloid cells, dendritic cells, and a subpopulation of T and B lymphocytes (1, 3). Mouse LMIR2 has the characteristics of an activatory molecule capable of inducing cellular activation and effector function in most cells and macrophages (3). The ligand for LMIR2 is presently unknown.
References
- Jackson, D.G. et al. (1992) Eur. J. Immunol. 22:1157.
- Clark, G.J. et al. (2001) Tissue Antigens 57:415.
- Clark, G.J. et al. (2002) J. Biol. Regul. Homeost. Agents 16:233.
- Daish, A. et al. (1993) Immunology 79:55.
Long Name
CD300 Antigen-Like Family Member C
Alternate Names
CLM-6, CLM6, CMRF35, IGSF16
Gene Symbol
CD300C
UniProt
Additional CD300c Products
Product Documents for Recombinant Human CD300c Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human CD300c Fc Chimera Protein, CF
For research use only
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