Recombinant Human CD39L3/ENTPD3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4400-EN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CD39L3/ENTPD3 protein
Gln44-Pro485, with a C-terminal 6-His tag
Gln44-Pro485, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln44 predicted
Predicted Molecular Mass
50 kDa
SDS-PAGE
65-80 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >70,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >70,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4400-EN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD39L3/ENTPD3
Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3), encoded by the ENTPD3 gene and also known as CD39L3, is an integral membrane protein with an extracellular active site (1). Recombinant human NTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-3 hydrolyzes the beta- and gamma-phosphate residues of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nucleotides, NTPDase-3 plays a role in the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen and prostate tissues (3).
References
- Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
- Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
- Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
Alternate Names
ATPDase, CD39L3, ENTPD3, HB6, NTPDase-3
Gene Symbol
ENTPD3
UniProt
Additional CD39L3/ENTPD3 Products
Product Documents for Recombinant Human CD39L3/ENTPD3 Protein, CF
Product Specific Notices for Recombinant Human CD39L3/ENTPD3 Protein, CF
For research use only
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