Recombinant Human CD40/TNFRSF5 Fc Alexa Fluor® 488 Protein

R&D Systems, part of Bio-Techne | Catalog # AFG1493

Fc Chimera His-tag
R&D Systems, part of Bio-Techne
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AFG1493-020
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Key Product Details

Learn more about FluorokinesTM Fluorescent-Labeled Proteins

Source

NS0

Accession #

P25942-1

Structure / Form

Disulfide-linked homodimer
Labeled with Alexa Fluor® 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Conjugate

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Bioactivity

View all CD40/TNFRSF5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD40/TNFRSF5 protein
Human CD40
(Glu21-Arg193)
Accession # P25942-1		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

47 kDa

SDS-PAGE

58-66 kDa, under reducing conditions.

Activity

Measured by flow cytometry for its ability to bind anti-human CD40/TNFRSF5 antibody conjugated beads. The concentration of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 (Catalog # AFG1493) that produces 50% of the binding response is 15.0‑150 ng/mL.

Scientific Data Images for Recombinant Human CD40/TNFRSF5 Fc Alexa Fluor® 488 Protein

Flow cytometry analysis for Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 staining on human CD40/TNFRSF5 antibody conjugated beads.

Streptavidin coated beads conjugated to Human CD40/TNFRSF5 Biotinylated Antibody (BAF632) were stained with the indicated concentrations of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 (Catalog # AFG1493).

Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 Protein (Catalog # AFG1493) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-66 kDa and 120-130 kDa, respectively.

Formulation, Preparation and Storage

AFG1493
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

  1. Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
  2. Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
  3. Eshel, D. et al. (2008) Mol. Immunol. 46:250.
  4. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  5. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  6. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  7. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  8. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  9. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  10. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  11. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  12. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  13. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  14. Hassan, G.S. et al. (2012) Immunobiology 217:521.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Gene Symbol

CD40

UniProt

P25942-1

Additional CD40/TNFRSF5 Products

Product Documents for Recombinant Human CD40/TNFRSF5 Fc Alexa Fluor® 488 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CD40/TNFRSF5 Fc Alexa Fluor® 488 Protein

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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