Recombinant Human CD46 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10256-CD

R&D Systems, part of Bio-Techne
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10256-CD-050
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Key Product Details

Source

HEK293

Accession #

P15529-11

Conjugate

Unconjugated

Applications

Bioactivity

View all CD46 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD46 protein
Cys35-Asp328, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys35

Predicted Molecular Mass

34 kDa

SDS-PAGE

40-67 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD46 is immobilized at 5 μg/mL (100 μL/well), the concentration Human C4b that produces 50% of the optimal binding response is 2-12 μg/mL.

Scientific Data Images for Recombinant Human CD46 His-tag Protein, CF

Recombinant Human CD46 His-tag Protein Binding Activity

Recombinant Human CD46 His-tag Protein Binding Activity

When Recombinant Human CD46 (Catalog # 10256-CD) is immobilized at 5 μg/mL (100 μL/well), it binds to Human C4b with an ED50 of 2‑12 μg/mL.
Recombinant Human CD46 His-tag Protein SDS-PAGE

Recombinant Human CD46 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CD46 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-67 kDa.

Formulation, Preparation and Storage

10256-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD46

CD46, also known as MCP (Membrane cofactor protein), is a type I membrane protein that functions as a cofactor for Complement Factor I­mediated inactivation of complement components C3b and C4b (1, 2). Mature human CD46 consists of a 309 amino acid (aa) extracellular domain (ECD) with four short consensus repeats (SCR) and a Ser/Thr/Pro-enriched region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain (1). Within the ECD, human CD46 shares 50% aa sequence identity with mouse and rat CD46. CD46 is commonly expressed in four distinct isoforms, and all isoforms have conserved complement control protein domains which harbor the C3b and C4b binding sites (3). CD46 is expressed on all nucleated cells (4). CD46 participates in the maintenance of immune homeostasis and the induction of protective T cell immunity, and it could extend towards key processes in development and cell metabolism (5).

References

  1. Liszewski, M.K. et al. (1991) Annu. Rev. Immunol. 9:431.
  2. Barilla-LaBarca, M.L. et al. (2002) J. Immunol. 168:6298.
  3. Yamamoto, H. et al. (2013) Intl. J. Biochem. Cell Biol. 45:2808.
  4. Andrews, P.W. et al. (1985) Ann. Hum. Genet. 49:31.
  5. Kolev, M. et al. (2013) Semin. Immunol. 25:12.

Alternate Names

CD46, MCP

Entrez Gene IDs

4179 (Human); 17221 (Mouse)

Gene Symbol

CD46

UniProt

P15529-11

Additional CD46 Products

Product Documents for Recombinant Human CD46 His-tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human CD46 His-tag Protein, CF

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