Recombinant Human CD6 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11288

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P30203.3

Conjugate

Biotin

Applications

Bioactivity

View all CD6 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD6 protein
Human CD6
(His18-Glu398)
Accession # P30203.3		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His18

Predicted Molecular Mass

43 kDa

SDS-PAGE

82-91 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 7187-AL) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD6 His-tag Avi-tag (Catalog # AVI11288) binds with an ED50 of 0.300-3.00 µg/mL.

Scientific Data Images for Recombinant Human CD6 His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human CD6 His-tag Avi-tag Protein Binding Activity.

When Recombinant Human ALCAM/CD166 Fc Chimera (7187-AL) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD6 His-tag Avi-tag Protein (Catalog # AVI11288) binds with an ED50 of 0.300-3.00 µg/mL.

Biotinylated Recombinant Human CD6 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD6 His-tag Avi-tag Protein (Catalog # AVI11288) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 82-91 kDa.

Formulation, Preparation and Storage

AVI11288
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. CD6 is a 668 amino acid protein with a 24 amino acid predicted signal sequence, a 374 amino acid extracellular domain, and a 244 amino acid transmembrane region. The 626 amino acid murine homolog has also been identified. The human and murine proteins share 70% amino acid identity over their full-lengths.The role of CD6 has not been fully elucidated. However, it appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogeneic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. The presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cells and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins. However, the signaling pathways have not been elucidated. Our Avi-tag Biotinylated human CD6 His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Gangemi, R.M. et al. (1989) J. Immunol. 143:2439.
  2. Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
  3. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
  4. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
  5. Whitney, G. et al. (1995) Mol. Immunol. 32:89.
  6. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
  7. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  8. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
  9. Pawelec, G. and H.J. Buhring (1991) Hum. Immunol. 31:165.
  10. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
  11. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
  12. Singer, N.G. et al. (1996) Immunology 88:537.
  13. Aruffo, A. et al. (1997) Immunol. Today 18:498.

Alternate Names

CD6

Entrez Gene IDs

923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)

Gene Symbol

CD6

UniProt

P30203.3 (Human)

Additional CD6 Products

Product Documents for Recombinant Human CD6 His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human CD6 His-tag Avi-tag Protein, CF

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